Key Trait Screening on Global Wheat Accessions using KASP Genotyping Markers

Key Trait Screening on Global Wheat Accessions using KASP Genotyping Markers

11 Jan 2016

This application note offers an example of how KASP genotyping assays for QTL can be used to survey potential breed-lines for crop development using these publicly available markers. Greater availability of high-throughput genotyping methods has lead to an explosion of single nucleotide polymorphism (SNP) sequence data from plant genomes. This has enabled the discovery of large numbers of potential SNP markers for varietal improvement. The use of KASP genotyping as a cost-effective, efficient tool for introgression of important traits has now been widely published.

sbeadex Nucleic Acid Extraction Kits

LGC Biosearch Technologies

Our proprietary sbeadex magnetic microparticles are designed to deliver high yields of high quality DNA, and have long enabled high throughput automated processing.   sbeadex™ magnetic microparticles offer two key features which enable the extraction and purification of high quality DNA preparations: A novel two step binding mechanism via a patented adapter enables binding of nucleic acids more specifically. Final washes with pure water to deliver nucleic acids in high yield, purity and quality.   Features: Development of customised protocols to your requirements Tailor-made extractions like modified lysis or eluation steps according to user requirements Extraction speed optimisation - quicker results Highly flexible batch sizes   Technical advantages: Final washes with pure water to deliver nucleic acids in high yield, purity and quality No potential PCR inhibitors in wash buffers - e.g. organic solvents, salts, polyethyleneglycol Applicable for most popular robotic platforms (f.e. Hamilton, TECAN or KingFisher)   Available sbeadex kits: sbeadex plant kit sbeadex blood kit sbeadex plasmid kit sbeadex forensic kit sbeadex tissue kit sbeadex livestock kit sbeadex pathogen kit   The sbeadex technologies are suitable for high throughput automation and we have substantial experience developing sbeadex kits and protocols for use with a wide range of automation systems.

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Kleargene™ Spin Plates for Nucleic Acid Extraction

LGC Biosearch Technologies

Kleargene spin plates utilize a glass fibre solid support and are suited for manual or automated DNA extractions from small samples.   This includes samples such as plant seeds and tissue, animal tissue (rodent tails, etc...), blood, buccal swabs, buffy coat, saliva and more. Kits are available in both 96 and 384-well formats. The technology is optimally suited for nucleic acid extractions yielding between 100 ng to 10 µg of DNA.  

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