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Key Trait Screening on Global Wheat Accessions using KASP Genotyping Markers

LGC Diagnostics and Genomics - Rapid Genomics Genotyping Solutions

Key Trait Screening on Global Wheat Accessions using KASP Genotyping Markers

11 Jan 2016

This application note offers an example of how KASP genotyping assays for QTL can be used to survey potential breed-lines for crop development using these publicly available markers. Greater availability of high-throughput genotyping methods has lead to an explosion of single nucleotide polymorphism (SNP) sequence data from plant genomes. This has enabled the discovery of large numbers of potential SNP markers for varietal improvement. The use of KASP genotyping as a cost-effective, efficient tool for introgression of important traits has now been widely published.

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sbeadex Nucleic Acid Extraction Kits

LGC Diagnostics and Genomics - Rapid Genomics Genotyping Solutions

Our proprietary sbeadex magnetic microparticles are designed to deliver high yields of high quality DNA, and have long enabled high throughput automated processing.   sbeadex™ magnetic microparticles offer two key features which enable the extraction and purification of high quality DNA preparations: A novel two step binding mechanism via a patented adapter enables binding of nucleic acids more specifically. Final washes with pure water to deliver nucleic acids in high yield, purity and quality.   Features: Development of customised protocols to your requirements Tailor-made extractions like modified lysis or eluation steps according to user requirements Extraction speed optimisation - quicker results Highly flexible batch sizes   Technical advantages: Final washes with pure water to deliver nucleic acids in high yield, purity and quality No potential PCR inhibitors in wash buffers - e.g. organic solvents, salts, polyethyleneglycol Applicable for most popular robotic platforms (f.e. Hamilton, TECAN or KingFisher)   Available sbeadex kits: sbeadex plant kit sbeadex blood kit sbeadex plasmid kit sbeadex forensic kit sbeadex tissue kit sbeadex livestock kit sbeadex pathogen kit   The sbeadex technologies are suitable for high throughput automation and we have substantial experience developing sbeadex kits and protocols for use with a wide range of automation systems.

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Kleargene™ Spin Plates for Nucleic Acid Extraction

LGC Diagnostics and Genomics - Rapid Genomics Genotyping Solutions

Kleargene spin plates utilize a glass fibre solid support and are suited for manual or automated DNA extractions from small samples. This includes samples such as plant seeds and tissue, animal tissue (rodent tails, etc...), blood, buccal swabs, buffy coat, saliva and more. Kits are available in both 96 and 384-well formats. The technology is optimally suited for nucleic acid extractions yielding between 100 ng to 10 µg of DNA.  

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.Data AnalysisData analysis hardware and software is available to make data processing straight-forward yet powerful. Data software can be used for math and stats, technical graphing and image analysis. In addition, software is available for specific data analysis of electrophoresis, densitometry, ELISA and DNA sequencing.SNPs TechnologySingle nucleotide polymorphisms (SNPs) are individual base variations in a DNA sequence. SNPs are used in research to study predispositions to disease and drug discovery. Products for studying single nucleotide polymorphisms include SNP arrays and detection systems to detect polymorphisms, SNP typing systems for genotyping DNA, and PCR to amplify specific SNPs.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.AgricultureAgriculture research focuses on improving food production, crop management, and sustainability. This includes plant breeding, pest control, and soil health studies. Explore agriculture research tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Data LoggingHigh ThroughputHigh throughput experiments allow the simultaneous processing of several samples. This parallelization reduces the cost per experiment and increases reproducibility and output volume of data.Process OptimizationProcess optimization involves improving the efficiency, cost-effectiveness, and quality of processes in various industries, including manufacturing, pharmaceuticals, and biotechnology. Explore process optimization solutions in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Phenotypic ScreeningPhenotypic screening assesses cellular responses to compounds, enabling drug discovery and target identification. This technique is pivotal in finding effective treatments for complex diseases. Discover phenotypic screening platforms and tools with peer-reviewed comparisons and pricing in our directory.SNP GenotypingBiomarker DiscoveryClinical biomarkers refer to substances related to known medical conditions that can be accurately measured <i>in vitro</i>. Biomarkers can be used to diagnose presence of a disease and indicate disease severity. The discovery of new biomarkers is incredibly valuable in the field of diagnostics.

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Key Trait Screening on Global Wheat Accessions using KASP Genotyping Markers