Member since: 2022
Good quality and quantity was achieved
Application Area: Transcript analysis for specific genes
"The product is very user friendly, the quality of RNA is great and quantity as well. The product is worth of money for various research perposes."
Member since: 2018
Organization: National Chung Chung Hsing University Taichung
Application Area:RNA isolation kit
"Good product and very easy to use. Can extract RNA using its protocols very simply."
The RNAqueous®-Micro Kit is used for phenol-free total RNA isolation from micro-sized samples (e.g., from <500,000 cultured cells, from few as 10 laser capture microdissected cells, or from ≤10 mg of tissue) using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.
DNA-free™ DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR.
This kit provides:
How it works:
Samples are solubilized in a powerful chaotropic solution, and RNA is purified on a glass fiber filter cartridge specifically designed for very small samples. A simple modification of the protocol allows recovery of both large and small RNA species including tRNAs, 5S rRNA, and microRNAs. The RNA is eluted in a minimal volume of 20 µL. Residual genomic DNA is efficiently removed using the DNA-free™ system included in the kit. The RNAqueous®-Micro Kit contains enough reagents for 50 RNA isolations of DNA-free RNA.