RNAqueous Micro Total RNA Isolation Kit by Thermo Fisher Scientific
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Status:
Reviewer
Member since: 2022
Organization: UMINHO
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- Ease of use
- After sales service
- Value for money
Good quality and quantity was achieved
Application Area: Transcript analysis for specific genes
"The product is very user friendly, the quality of RNA is great and quantity as well. The product is worth of money for various research perposes."
Review date: 31 Oct 2022 | RNAqueous Micro Total RNA Isolation Kit
Status:
Reviewer Member since: 2018
Organization: National Chung Chung Hsing University Taichung
-
- Ease of use
- After sales service
- Value for money
Great results.
Application Area:RNA isolation kit
"Good product and very easy to use. Can extract RNA using its protocols very simply."
Review date: 21 Mar 2020 | RNAqueous Micro Total RNA Isolation Kit
The RNAqueous®-Micro Kit is used for phenol-free total RNA isolation from micro-sized samples (e.g., from <500,000 cultured cells, from few as 10 laser capture microdissected cells, or from ≤10 mg of tissue) using a guanidinium-based lysis/denaturant and glass fiber filter separation technology.
DNA-free™ DNA removal reagents are included in the kit, making the isolated RNA especially suitable for RT-PCR.
This kit provides:
- Highly concentrated RNA—for increased sensitivity in downstream assays
- Isolation of high-quality RNA from micro-scale samples
- Reagents/protocol for RNA recovery from LCM samples
How it works:
Samples are solubilized in a powerful chaotropic solution, and RNA is purified on a glass fiber filter cartridge specifically designed for very small samples. A simple modification of the protocol allows recovery of both large and small RNA species including tRNAs, 5S rRNA, and microRNAs. The RNA is eluted in a minimal volume of 20 µL. Residual genomic DNA is efficiently removed using the DNA-free™ system included in the kit. The RNAqueous®-Micro Kit contains enough reagents for 50 RNA isolations of DNA-free RNA.
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