RevertAid First Strand cDNA Synthesis Kit by Thermo Fisher Scientific

Manufacturer Thermo Fisher Scientific  |  Available Worldwide
5.0
/
5.0
  |  2 reviews
Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates


RevertAid First Strand cDNA Synthesis Kit by Thermo Fisher Scientific product image
RevertAid First Strand cDNA Synthesis Kit

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Average Rating: 5.0
2 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2020

  • Organization: NATIONAL DAIRY RESEARCH INSTITUTE



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Very good results.
Rating: 5.0

  • Application Area: Sequencing

"Very effective, easy to handle and high efficiency."

Review date: 21 Jun 2020 | RevertAid First Strand cDNA Synthesis Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Faculty Of Biology University Of Belgrade



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Share this ReviewShare this Review on TwitterShare this Review
Great results with cDNA.
Rating: 5.0

  • Application Area:Reverse transcription

"Excellent product for RT reaction, must have in any lab."

Review date: 21 Jun 2019 | RevertAid First Strand cDNA Synthesis Kit

Thermo Scientific RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit uses RevertAid Reverse Transcriptase (RT), a recombinant M-MuLV RT which maintains activity at 42-50°C and is suitable for synthesis of cDNA up to 13 kb. RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA templates from degradation.

The RevertAid First Strand cDNA Synthesis Kit is supplied with both oligo(dT)18 and random hexamer primers. The oligo(dT)18 primer anneals selectively to the poly(A) tail of mRNA. Random hexamer primers do not require the presence of the poly(A) tail, therefore, they can be used for transcription of the 5'-end regions of mRNA or cDNA synthesis of RNA species lacking a poly(A) tail (e.g., microRNAs). Gene-specific primers may also be used with the kit.

Highlights

  • Full-length first strand cDNA up to 13 kb
  • Optimum reaction temperature 42°C
  • Complete kit—all the components for the RT reaction are included

Applications

  • First strand cDNA synthesis for RT-PCR and RT-qPCR
  • Construction of full length cDNA libraries
  • Antisense RNA synthesis

For Research Use Only. Not for use in diagnostic procedures.

Product Overview

RevertAid First Strand cDNA Synthesis Kit by Thermo Fisher Scientific product image

RevertAid First Strand cDNA Synthesis Kit

Manufacturer Thermo Fisher Scientific  |  Available Worldwide

5.0 / 5.0 | 2 reviews