This toolkit of alternative Flash media provides faster, cleaner separations by eliminating common problems encountered in normal-phase purification of basic compounds.
The Redisep Basic Compound Purification Kit includes:
By using these alternative media, drug discovery researchers can avoid time-consuming extra steps in the purification of high-pKa compounds such as amines and nitrogen-containing heterocycles: Peak streaking and tailing, resulting in multiple fractions and overlapping fractions during chromatographic purification. Time-consuming extra steps to remove residues of non-volatile mobile-phase modifiers (TEA or NH 4 OH) remaining in the sample after evaporation of the DCM and methanol chromatography solvents.
Additional time spent swapping and priming the solvents on the flash chromatography system – typically to change from a hexane/ethyl acetate to a dichloromethane/ methanol solvent system. A purge and solvents switch after the run may also be needed. In addition to the alternative-media Flash columns and TLC plates, the kit includes a comprehensive guide to purification of Basic compounds.