Renilla Reniformis (sea Pansy)
Escherichia coli (E.coli)
C-terminal 6x histidine tag
Lyophilized. Renilla luciferase protein was constituted in a 0.2 µm filtered phosphate-buffered saline (1x PBS) before lyophilized.
ELISA, Western blotting, Dot Blotting, Immunogen, Protein Array, Function Assay, etc.
Using the Renilla Lucifearse assay kit (Promega), the measured net Relative Light Unit (RLU) was approximately 5 X 1010
Stability & Storage
Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS.
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.