REAlease^® Releasable Antibodies by Miltenyi Biotec B.V. & Co. KG

REAlease^® Releasable Antibodies are made from antibody fragments that are characterized by low epitope binding affinities. Antibody fragments and fluorochromes form the so called “REAlease Fluorochrome Complex”  which shows high-avidity cell binding properties, comparable to conventional antibodies.

This novel and innovative conjugation chemistry allows for fast removal of the REAlease Fluorochrome Complex from the cell surface by adding the REAlease Release Reagent after cell sorting. This leads to disruption of the complex and thereby results in monomerization of the antibody fragments.

Due to their engineered low binding affinity, they spontaneously dissociate from the cell surface. As a result, label-free cells can be obtained after cell sorting providing maximal flexibility for a wide range of applications.

The benefits

• Epitope of a marker becomes available again for downstream applications that require free epitopes (e.g. cell culture experiments and cell analysis)
• Fluorescence channel becomes available again for relabeling (e.g. microscopy applications)
• Sequential flow sorting cycles for the isolation of specific cell subsets