RapiDxFire™ Thermostable Reverse Transcriptase by LGC, Biosearch Technologies

Manufacturer LGC, Biosearch Technologies  |  Available Worldwide
Originally identified in hot springs, this robust bacteriophage enzyme exhibits increasingly high activity at elevated temperatures (~60% activity remains after 10 min at 90°C) and maintains its core activity after >3 months storage at 20-25°C. This enzyme lacks RNAse H and 3’ -> 5’ exonuclease activity and efficiently synthesizes short cDNA fragments (≤ 1 Kb). 


RapiDxFire™ Thermostable Reverse Transcriptase by LGC, Biosearch Technologies product image
RapiDxFire™ Thermostable Reverse Transcriptase
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Originally identified in hot springs, this robust bacteriophage enzyme exhibits increasingly high activity at elevated temperatures (~60% activity remains after 10 min at 90⁰C) and maintains its core activity after >3 months storage at 20-25⁰C. This enzyme lacks RNAse H and 3’ -> 5’ exonuclease activity and efficiently synthesizes short cDNA fragments (≤ 1 Kb). Like other reverse transcriptases, RapiDxFire RT possesses DNA polymerase activity but lacks 5′ -> 3′ exonuclease activity. Supplied in a glycerol-free, Triton™ X-100-free storage buffer, the enzyme formulation can be further optimized for downstream lyophilization.

A truly thermostable reverse transcriptase for fast synthesis of short cDNA (< 1 Kb)

  • Extremely active at high temperatures (55 to 80°C): Improves specificity of cDNA Synthesis from diverse RNA templates
  • Sensitive: Detects ≤100 copies of RNA in two-step RT-qPCR assays
  • Short reaction time (5 minutes or less): Streamlined RT-qPCR workflow and faster time to results
  • Stable at room temperature (> 3 months): Simplifies setup on automation decks (no cold storage required) and enables use in environments with limited cold-storage
  • Lyo-compatible: Enzyme formulation is free of glycerol -and other components- that are known to interfere with downstream lyophilization
  • Batch to batch reproducibility: Manufactured in an ISO 13485-certified facility