Originally identified in hot springs, this robust bacteriophage enzyme exhibits increasingly high activity at elevated temperatures (~60% activity remains after 10 min at 90⁰C) and maintains its core activity after >3 months storage at 20-25⁰C. This enzyme lacks RNAse H and 3’ -> 5’ exonuclease activity and efficiently synthesizes short cDNA fragments (≤ 1 Kb). Like other reverse transcriptases, RapiDxFire RT possesses DNA polymerase activity but lacks 5′ -> 3′ exonuclease activity. Supplied in a glycerol-free, Triton™ X-100-free storage buffer, the enzyme formulation can be further optimized for downstream lyophilization.
A truly thermostable reverse transcriptase for fast synthesis of short cDNA (< 1 Kb)