QX200™ Droplet Digital PCR System by Bio-Rad
Status:
Reviewer
Member since: 2022
Organization: Frontera Therapeutics
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- Ease of use
- After sales service
- Value for money
My choice of instrument!
Application Area: Analyzing genome copies
"This is my first time to use this system. I was amazed by how easy and robust this system is - the duplicated wells gave very tight results. The reproducibility was impressive."
Review date: 15 Nov 2022 | QX200™ Droplet Digital PCR System
Status:
Reviewer Member since: 2016
Organization: Biodonostia
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- Ease of use
- After sales service
- Value for money
Very useful platform. Easy to handle
Application Area:Quantification of fungi and bacteria in grape samples
"Digital PCR is key in our lab when analysing certain samples. QX200 system is easy to use and allows the analysis of a 96 plate in a very short time. I wish reactives were cheaper!"
Review date: 29 Sep 2022 | QX200™ Droplet Digital PCR System
Status:
Reviewer Member since: 2019
Organization: University of Swabi
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- Ease of use
- After sales service
- Value for money
Yes it is working very well. It is the need of today for any life science laboratory
Application Area:Molecular biology
"The product is very excellent. It's very reasonable in price as well as function very well from past few years. "
Review date: 24 Jan 2022 | QX200™ Droplet Digital PCR System
Status:
Advanced Reviewer Member since: 2018
Organization: Bioinformatics researcher at MAJU Islamabad
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- Ease of use
- After sales service
- Value for money
Easy to use.
Application Area:Analyze the PCR efficiency
"Absolute quantification — provides an absolute count of target DNA copies per input sample without the need for standard curves. Highest precision and sensitivity — enriched template concentration in target-positive droplets allows higher precision and sensitivity than real-time PCR. High throughput — simple and easy-to-use workflow with 96-sample throughput does not require multiple dilution steps. Removal of PCR efficiency bias — error rates are acceptable."
Review date: 20 May 2021 | QX200™ Droplet Digital PCR System
Status:
Reviewer Member since: 2021
Organization: Medigene Immunotherapies GmbH
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- Ease of use
- After sales service
- Value for money
Great device, robust results, even under GCLP.
Application Area:Develop safety assays
"The device is very effective and robust. We developped VCN assay for release assay under GCLP conditions. Easy to use, linearity, accuracy, precision, LOB, LOD, LOQ, LLOQ, ULOQ, all parameters worked fine for us"
Review date: 23 Feb 2021 | QX200™ Droplet Digital PCR System
Status:
Expert Reviewer Member since: 2016
Organization: SC Department of Health and Environmental Control
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- Ease of use
- After sales service
- Value for money
Excellent product for gene quantification.
Application Area:Analyze genes and quantify with high reproducibility
"This digital PCR system is very useful for generating several droplets from a single sample. High reproducibility and great quantification. It is very useful for detecting the sensitivity of the gene. The quality of the product is very high and reproducible and highly recommendable for gene expression analysis and transcriptomics experiments."
Review date: 11 Dec 2020 | QX200™ Droplet Digital PCR System
Status:
Reviewer Member since: 2020
Organization: Novartis
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- Ease of use
- After sales service
- Value for money
Great instrument, can't live without this instrument.
Application Area:AAV samples for titer
"I have two QX200 ddPCR systems in my lab. We use these instruments day in, day out for analysis of our AAV samples from Manufacturing and PD. They do a great job providing the throughput."
Review date: 20 Nov 2020 | QX200™ Droplet Digital PCR System
Status:
Reviewer Member since: 2020
Organization: DNAwesome
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- Ease of use
- After sales service
- Value for money
I liked the tool, but hoped it would be little cheaper.
Application Area:Biotechnology and genetics
"It's great and gives great results. It's quite expensive. I also faced a problem with shipping."
Review date: 15 May 2020 | QX200™ Droplet Digital PCR System
Status:
Advanced Reviewer Member since: 2015
Organization: Temple University School of Medicine
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- Ease of use
- After sales service
- Value for money
Now we are using predominantly ddPCR for all our projects!
Application Area:Gene expression, miRNA changes
"We are getting very reproducible data, accurate results, easy to use, absolute numbers using very small amounts of RNA/cDNA."
Review date: 18 Nov 2019 | QX200™ Droplet Digital PCR System
Status:
Reviewer Member since: 2019
Organization: Microbiologics, Inc.
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- Ease of use
- After sales service
- Value for money
Huge asset to our lab!
Application Area:Quantifying inactivated microbial cultures
"This system has helped us improve the accuracy of our quantification and ensure repeatable results across operators."
Review date: 14 Nov 2019 | QX200™ Droplet Digital PCR System
Status:
Reviewer Member since: 2019
Organization: Biocartis
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- Ease of use
- After sales service
- Value for money
Makes live cell analysis very easy.
Application Area:Determine the % of mutant copies
"It's very easy to use. You can detect small concentrations of mutant target within a pool of wild type DNA. We have had good services."
Review date: 20 Aug 2019 | QX200™ Droplet Digital PCR System
Status:
Reviewer Member since: 2018
Organization: Hospital de Puerto Montt
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- Ease of use
- After sales service
- Value for money
The future!
Application Area:cfDNA analysis
"Still in validation, however, the results are promising."
Review date: 08 May 2019 | QX200™ Droplet Digital PCR System
Status:
Reviewer Member since: 2018
Organization: Hospital de Puerto Montt
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- Ease of use
- After sales service
- Value for money
The platform opens a new windows on clinical diagnostic on cancer.
Application Area:Clinical cancer diagnostic
"The platform opens a new windows on clinical diagnostic on cancer."
Review date: 20 Dec 2018 | QX200™ Droplet Digital PCR System
Status:
Advanced Reviewer Member since: 2015
Organization: Temple University School of Medicine
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- Ease of use
- After sales service
- Value for money
Great and accurate results
Application Area:Analyze gene and miRNA copy number
"We achieved very high quality results and spent little time."
Review date: 20 Jul 2018 | QX200™ Droplet Digital PCR System
Status:
Reviewer Member since: 2018
Organization: IRCCS-CROB
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- Ease of use
- After sales service
- Value for money
Recommended!
Application Area:Cancer Biology; Onco-Genetic
"It's easy to use. RED performed by quantitative-polymerase chain reaction (qRT-PCR) were compared to ddPCR. The results are comparable with those obtained using qRT-PCR."
Review date: 11 Apr 2018 | QX200™ Droplet Digital PCR System
Status:
Advanced Reviewer Member since: 2016
Organization: Florida State University
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- Ease of use
- After sales service
- Value for money
Expensive, but is definitely worth the money
Application Area:Absolute quantification of DNA copy number, CNV, quantitative PCR to detect small change
"Since we only used demo unit, I haven't experienced after purchase care. However, I am totally happy with their customer support and technical support. This equipment is expensive but has many applications and is definitely worth money. We will try to purchase it within a year and applying for an equipment grant."
Review date: 09 Apr 2018 | QX200™ Droplet Digital PCR System
Status:
Expert Reviewer Member since: 2015
Organization: Bharat Serums and Vaccines Ltd
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- Ease of use
- After sales service
- Value for money
Great equipment
Application Area:Used for analyzing biological samples
"A very intelligent machine. Requires very low volumes of sample. highly sensitive and quite fast."
Review date: 21 Mar 2018 | QX200™ Droplet Digital PCR System
Status:
Reviewer Member since: 2016
Organization: University of Pittsburgh
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- Ease of use
- After sales service
- Value for money
Good product, easy to use, reliable.
Application Area:Pittsburgh
"Easy to use, good service, good commensurable parts."
Review date: 03 Mar 2017 | QX200™ Droplet Digital PCR System
Status:
Advanced Reviewer Member since: 2017
Organization: University of Rajarata, Sri Lanka
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- Ease of use
- After sales service
- Value for money
It gives precise, absolute target quantification as same as manufacturer stated.
Application Area:Dengue research and diagnostic
"It gives precise, absolute target quantification as manufacturer state. It’s amazing."
Review date: 03 Mar 2017 | QX200™ Droplet Digital PCR System
Status:
Reviewer Member since: 2017
Organization: University of South Alabama
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- Ease of use
- After sales service
- Value for money
A nice advancement in our capabilities.
Application Area:Cancer Research
"It allows for the detection of small variations/changes, which is very beneficial. Also, I find that the instrument helps analyze complex mixtures, better than the traditional q-PCRS. I am also less worried about buffer composition."
Review date: 03 Mar 2017 | QX200™ Droplet Digital PCR System
Status:
Reviewer Member since: 2016
Organization: National Institute of Biology
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- Ease of use
- After sales service
- Value for money
Great machine for nucleic acid quantification.
Application Area:Absolute quantification of viruses
"Easy to use and robust enough that even a beginner can get the same results as an expert. Can be applied to various fields of application. If droplet reading was a bit faster the machine would be absolutely perfect."
Review date: 21 Dec 2016 | QX200™ Droplet Digital PCR System
Status:
Reviewer Member since: 2001
Organization: Global IT Solutions
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- Ease of use
- After sales service
- Value for money
Bio-rad ddPCR highly effective.
Application Area:Sensitive detection of viral rna
"Effective in detecting viral mutations. High quality results with easy use. Highly recommended. Great company with extensive support. Cost effective."
Review date: 30 Mar 2016 | QX200™ Droplet Digital PCR System
Status:
Reviewer Member since: 2016
Organization: Cleveland Clinic Foundation
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- Ease of use
- After sales service
- Value for money
Simple but efficient system
Application Area:Gene quant
"It provides an absolute count of target DNA copies per input sample without the need for standard curves which makes it easier to use compared to real-time PCR in which there may be variations due to reference gene amplification. Also simple and easy-to-use workflow with 96-sample throughput does not require multiple dilution steps. "
Review date: 24 Mar 2016 | QX200™ Droplet Digital PCR System
Status:
Reviewer Member since: 2016
Organization: Horizon Discovery
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- Ease of use
- After sales service
- Value for money
A must in every lab.
Application Area:genotyping
"Very easy to operate, providing excellent results. Very reliable as well!"
Review date: 24 Mar 2016 | QX200™ Droplet Digital PCR System
Status:
Reviewer Member since: 2014
Organization: King AbdulAziz University
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- Ease of use
- After sales service
- Value for money
Application Area:MDx
"When your qPCR detecting a Cq over 32, the best technique is to use the highly sensitive QX 200 ddPCR."
Review date: 06 Oct 2015 | QX200™ Droplet Digital PCR System
Status:
Reviewer Member since: 2015
Organization: Lund University
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- Ease of use
- After sales service
- Value for money
Application Area:Plasma
"Very useful product specially CNV and rare mutation analysis"
Review date: 21 Sep 2015 | QX200™ Droplet Digital PCR System
Status:
Reviewer Member since: 2015
Organization: AltraTech Ltd
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- Ease of use
- After sales service
- Value for money
Application Area:PCR
"I was very happy with the results I achieved with the Bio-Rad QX200™ Droplet Digital™ PCR System and found it easy-to-use."
Review date: 25 Mar 2015 | QX200™ Droplet Digital PCR System
Status:
Reviewer Member since: 2014
Organization: University of Michigan
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- Ease of use
- After sales service
- Value for money
Application Area:We use it for two purposes: Genotyping (copy number) and gene expression
"So far, the droplet technology has been pretty straightforward to use and teach to others. It has thus far replicated several differential gene expression and copy number studies in our lab."
Review date: 17 Nov 2014 | QX200™ Droplet Digital PCR System
Status:
Reviewer Member since: 2013
Organization: Autonomous University of Tamaulipas
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- Ease of use
- After sales service
- Value for money
Application Area:Polymorphism
"It worked well but it needs a better support life. Without it, it doesn't work well enough. I would recommend to other scientists."
Review date: 29 Nov 2013 | QX200™ Droplet Digital PCR System
Use the QX200 Droplet Digital PCR (ddPCR™) system, the most precise and sensitive PCR solution, for absolute quantification of target DNA molecules.
Droplet Digital PCR (ddPCR™) is Bio-Rad's unique digital PCR technology. With unrivaled precision, ddPCR provides absolute quantification of target DNA or RNA molecules without the use of standard curves. ddPCR addresses the lack of scalable and practical technologies for digital PCR implementation.
The new QX200 ddPCR system puts this powerful technology in your hands, ready to unveil new worlds of research at previously unattainable levels.
The QX200 droplet generator partitions the ddPCR reaction mix into 20,000 nanoliter-sized droplets. After PCR on a thermal cycler, droplets from each sample are analyzed individually on the QX200 droplet reader. Droplets are sipped, and the singulator unpacks the emulsified droplets and streams them in single file past a two-color optical detection system in a serial manner. Up to 96 samples can be processed per run.
The PCR-positive and PCR-negative droplets are counted to provide absolute quantification of target DNA in digital form. Alternatively, amplified products can be extracted from droplets following PCR for downstream applications, such as sequencing or cloning.
- Featured at Bio-Rad AACR 2014
- Winner of the SelectScience Scientists' Choice Awards Best New Life Sciences Product of 2013
Features
- Flexible digital PCR chemistry – optimized for TaqMan hydrolysis probe and EvaGreen applications
- Reduced bias from amplification efficiency and PCR inhibitors
- Flexible assay setup – scalable for high sensitivity or high throughput
- Convenient assay design – standard curves are not required
- Simple workflow with 96-sample throughput
Applications
- Cancer biomarker studies and copy number variation
- Pathogen detection
- Next generation sequencing
- Gene expression analysis
- Environmental monitoring
- Food testing
For extended product descriptions and detail specification information, please visit Bio-Rad.com.
Product Overview
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