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Quality control solutions for cell and gene therapy

Bio-Rad
14 Aug 2022

Bio-Rad offers a comprehensive portfolio of ddPCR Assays and Kits for numerous applications, including mutation detection, copy number determination, genome edit detection, gene expression analysis, Mycoplasma detection, multiplex mutation screening, residual DNA quantification, and library quantification.

This brochure provides greater detail on some of the quality control solutions available for cell and gene therapy.

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ddPCR Cell and Gene Therapy Assays

Bio-Rad

Bio-Rad’s ddPCR Cell and Gene Therapy (CGT) Assays are custom ddPCR-optimized assays generated by the Cell and Gene Therapy Design Engine for your target Gene of Interest. These assays offer the ease and accuracy of ddPCR technology and come in multiple fluorophores to enable multiplexed Cell and Gene Therapy applications, including Viral Titer and Vector Copy Number measurements. Use the ddPCR CGT Assays individually or multiplex them with the Vector Backbone Assays to precisely measure the concentration of the therapeutic gene in your sample. Features and Benefits Quick assay design through the Cell and Gene Therapy Design Engine, saving you valuable time to focus on important experiments Robust assay design, optimized specifically for ddPCR technology Six probe fluorophore options to enable multiplexing Vector Backbone Assays available for multiplexing In-silico multiplexing checks (wet-lab validation recommended) Certificate of Analysis provided with each ddPCR CGT Assay order Assay sequence provided with select catalog numbers Applications and Uses Viral Vector Genome Titer Measurement Vector Copy Number Determination Gene Therapy Development and Manufacturing CAR-T Cell Therapy Development and Manufacturing

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QX200™ Droplet Digital PCR System

Bio-Rad

Droplet Digital PCR System, includes droplet generator, droplet reader, laptop computer, software, associated component consumables, for EvaGreen or probe-based digital PCR applications

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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Bioprocessing / FermentationBioprocessing is the use of biological materials to perform commercial, scientific or medical research processes. Biological materials used include cells, enzymes and organisms. Usually bioprocessing requires a batch or continuous bioreactor such as a fermentor or cell culture system. The advantages of using a reactor include high productivity, easy configuration, adjustable values and automation.DNA / RNA QuantificationDetection and quantification of nucleic acids is important in molecular biology, cloning, expression, forensics and clinical diagnostics. Nucleic acids can be detected by labeling with colorimetric, fluorescent or radio labels and using in situ hybridization kits to identify specific sequences. Multiple nucleic acids can be detected and quantified at once using RNA / DNA detection beads or RNA / DNA microarrays. Find the best DNA / RNA Quantification products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.DNA QuantificationDNA quantification is the process of measuring the amount of DNA in a sample. It is vital in genomics, molecular biology, and biotechnology, helping to ensure accurate experiments and data analysis. Explore DNA quantification tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.DNADeoxyribonucleic Acid (DNA) is the main component of chromosomes and the carrier of genetic information of living organisms. Find out here about PCR, NGS, ChIP-Seq, gel imaging, and many other techniques which can be used for the analysis of DNA.

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