ddPCR Cell and Gene Therapy Assays
Bio-Rad’s ddPCR Cell and Gene Therapy (CGT) Assays are custom ddPCR-optimized assays generated by the Cell and Gene Therapy Design Engine for your target Gene of Interest. These assays offer the ease and accuracy of ddPCR technology and come in multiple fluorophores to enable multiplexed Cell and Gene Therapy applications, including Viral Titer and Vector Copy Number measurements. Use the ddPCR CGT Assays individually or multi…
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Bio-Rad’s ddPCR Cell and Gene Therapy (CGT) Assays are custom ddPCR-optimized assays generated by the Cell and Gene Therapy Design Engine for your target Gene of Interest. These assays offer the ease and accuracy of ddPCR technology and come in multiple fluorophores to enable multiplexed Cell and Gene Therapy applications, including Viral Titer and Vector Copy Number measurements. Use the ddPCR CGT Assays individually or multiplex them with the Vector Backbone Assays to precisely measure the concentration of the therapeutic gene in your sample.
Features and Benefits
- Quick assay design through the Cell and Gene Therapy Design Engine, saving you valuable time to focus on important experiments
- Robust assay design, optimized specifically for ddPCR technology
- Six probe fluorophore options to enable multiplexing
- Vector Backbone Assays available for multiplexing
- In-silico multiplexing checks (wet-lab validation recommended)
- Certificate of Analysis provided with each ddPCR CGT Assay order
- Assay sequence provided with select catalog numbers
Applications and Uses
- Viral Vector Genome Titer Measurement
- Vector Copy Number Determination
- Gene Therapy Development and Manufacturing
- CAR-T Cell Therapy Development and Manufacturing
Brochures
Quality control solutions for cell and gene therapy
Bio-Rad offers a comprehensive portfolio of ddPCR Assays and Kits for numerous applications, including mutation detection, copy number determination, genome edit detection, gene expression analysis, Mycoplasma detection, multiplex mutation screening, residual DNA quantification, and library quantification.
This brochure provides greater detail on some of the quality control solutions available for cell and gene therapy.
Comparing ddPCR with qPCR for the quantification of AAV vector genomes
Developing effective and reproducible gene therapies requires the use of sensitive and robust testing methods, such as Droplet Digital PCR, to validate the quality of the therapeutic product. Bio-Rad's portfolio of ddPCR Viral Quantification solutions offers an accurate, precise, and robust method to measure the viral titer in a sample.
In this whitepaper, Martin Lock, Director, Process Development Gene Therapy Program, University of Pennsylvania, shares data comparing an AAV genome titer assay based on ddPCR with a standard and an optimized qPCR assay for the titration of both single-stranded (ss) and self-complementary (sc) AAV genomes.
Droplet Digital PCR tips and tricks: ddPCR assay design
In this video, Bio-Rad provides tips and tricks used in ddPCR assay design. Watch now to explore topics including the availability of commercial PrimePCR ddPCR assays from Bio-Rad, common pitfalls, and recommendations for designing an effective ddPCR assay.
Droplet Digital PCR versus plate-based digital PCR
In this video, discover the advantages of Droplet Digital PCR (ddPCR) compared to plate-based digital PCR for the measurement of adeno-associated virus (AAV) viral titers. Hear how Bio-Rad’s ddPCR solution is designed to provide precise AAV viral titer measurements allowing you to validate the quality of your therapeutic products with confidence.
