Comparing ddPCR with qPCR for the quantification of AAV vector genomes

Bio-Rad’s ddPCR Cell and Gene Therapy (CGT) Assays are custom ddPCR-optimized assays generated by the Cell and Gene Therapy Design Engine for your target Gene of Interest. These assays offer the ease and accuracy of ddPCR technology and come in multiple fluorophores to enable multiplexed Cell and Gene Therapy applications, including Viral Titer and Vector Copy Number measurements. Use the ddPCR CGT Assays individually or multiplex them with the Vector Backbone Assays to precisely measure the concentration of the therapeutic gene in your sample. Features and Benefits Quick assay design through the Cell and Gene Therapy Design Engine, saving you valuable time to focus on important experiments Robust assay design, optimized specifically for ddPCR technology Six probe fluorophore options to enable multiplexing Vector Backbone Assays available for multiplexing In-silico multiplexing checks (wet-lab validation recommended) Certificate of Analysis provided with each ddPCR CGT Assay order Assay sequence provided with select catalog numbers Applications and Uses Viral Vector Genome Titer Measurement Vector Copy Number Determination Gene Therapy Development and Manufacturing CAR-T Cell Therapy Development and Manufacturing

Droplet Digital PCR System, includes droplet generator, droplet reader, laptop computer, software, associated component consumables, for EvaGreen or probe-based digital PCR applications