Member since: 2017
Organization: Kilimanjaro Clinical Research Institute
Wonderful product, easy on the purse strings as well.
Application Area: Molecular detection of pathogen DNA in clinical samples
"I've used the qPCR probe master-mix for detection and speciation of Brucella DNA in extracts from various clinical samples. Recently, in a bid to standardise protocols across different research facilities in our multi-cite control programs, we compared the performance of reaction master mix reagents provided by different distributors in over 4 countries. The Qiagen qPCR Probe kit (500, 1000, and 2,500) still performed the closest to our reference published protocols, on both pure and spiked sample controls, as well as genomic material extracted from clinical samples. Excellent reproducibility and standard curve consistency across all sites in all 4 countries evaluated."
QuantiTect Probe RT-PCR Kit
For one-step qRT-PCR using sequence-specific probes for gene expression analysis
Qiagen QuantiTect Probe RT-PCR Kit, 1000 x 50L rxns, RNA Targets Sample, One-step RT-PCR Reaction, Sequence-specific Probes, Ideal for Real-time Quantification of RNA Targets, For One-step qRT-PCR Using Sequence-specific Probes for Gene Expression Analysis, Includes 25mL 2x QuantiTect Probe RT-PCR Master Mix, 0.5mL QuantiTect RT Mix, 20mL RNase-free Water