Member since: 2019
Organization: University of Manchester
Saves time and gives great results.
Application Area: For qPCR of RNA samples
"We use the Qiagen range of gene globe to select primers for both rat and human samples. The use of these pre-optimized assays saves us a lot of time. For the most part, we have had very few issues and it enables fast analysis of candidate gene expression. In cases where any issues have been identified - either with primer batches or master mix - the support has been great, and concessions made where necessary. They are on the pricey side for primers, but for consistency and time saved optimising primer assays - we would definitely recommend!"
QuantiTect Primer Assays are genomewide, bioinformatically validated primer sets for use in SYBR Green-based real-time RT-PCR on any cycler. Assays are available for all genes from human, rat, mouse, and many other species. Each assay for a specific gene is supplied as a lyophilized mix of forward and reverse primers that can be easily reconstituted to obtain a 10x assay solution (reaction components for real-time RT-PCR need to be ordered separately). When used in combination with QuantiFast, QuantiTect, Rotor-Gene, or FastLane Kits for SYBR Green detection, QuantiTect Primer Assays guarantee highly specific and sensitive results in real-time RT-PCR that are comparable to probe-based detection.
Guaranteed results with genomewide, predesigned primers
~100% PCR efficiencies for reliable relative quantification
High sensitivity and specificity
Accurate quantification over a wide linear range
Time and cost savings with SYBR Green detection