Prometheus NT.48 for protein stability by NanoTemper Technologies

Manufacturer NanoTemper Technologies  |  Available Worldwide
4.9
/
5.0
  |  7 reviews
Prometheus NT.48 is a capillary-based instrument that measures the thermal stability and turbidity of a protein sample using dye-free nanoDSF (nano Differential Scanning Fluorimetry) technology. Use only 10 μl of sample to obtain high-resolution data that enables you to make better decisions about your proteins during every step of the development workflow.


Prometheus NT.48 for protein stability by NanoTemper Technologies product image
Prometheus NT.48 for protein stability
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Average Rating: 4.9
7 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2018

  • Organization: Advanced Enzymes Technologies Ltd.



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    3 out of 5
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A must-have for biophysical characterization of proteins.
Rating: 4.3

  • Application Area: Analyze protein samples

"This is a very useful instrument if you are into protein characterization. It gives you valuable information on stability, and behaviour of the protein under several different conditions. One can process many samples in a short time, hence this is good for screening purposes. The data obtained would otherwise take a long time with other methods. Therefore one can make quick decisions. The instrument is easy to use and the data is easy to analyze."

Review date: 19 Jun 2021 | Prometheus NT.48 for protein stability
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Syngene International



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great.
Rating: 5.0

  • Application Area:Protein sample analysis

"High quality and reproducible data, high throughput and best customer service."

Review date: 08 Jun 2020 | Prometheus NT.48 for protein stability
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: DuPont IB/ Genencor



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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This is a great instrument!
Rating: 5.0

  • Application Area:Formulations

"Gives us reproducible data, consistently. No sample prep is needed which saves time."

Review date: 07 Feb 2017 | Prometheus NT.48 for protein stability
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Sanofi R&D



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great instrument! Robust, easy to use, cost- and time-effective in operations!
Rating: 5.0

  • Application Area:Small molecules and Biologics projects in drug discovery research

"Prometheus is very reliable and easy to use instrument that is delivering very high quality results, for both small molecules and biologics results. Its use is extremely time- and cost-effective. This instrument is one of the most used in our multi-disciplinary teams where we apply great number of biophysical instruments and dealing with >60 companies/vendors"

Review date: 01 Dec 2016 | Prometheus NT.48 for protein stability
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Inst. for Stem Cell biology & Regenerative Medicine



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Wonderful instrument and easy usage
Rating: 4.7

  • Application Area:Tm of membrane proteins

"The Prometheus NT48 is a very convenient way for us to test the efficacy of various chemicals that affect membrane protein stability. The small sample volume and relatively high throughput of the system makes additive selection for membrane proteins very easy."

Review date: 01 Dec 2016 | Prometheus NT.48 for protein stability
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: 2bind GmbH



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Fast and precise information on formulation of therapeutic antibodies.
Rating: 5.0

  • Application Area:Preformulation of therapeutic antibodies

"Our company 2bind uses the nanoDSF to optimize the formulation of therapeutic antibodies. The Prometheus allows us to screen 48 buffer conditions with a bit more than an hour. The antibody can be used as it is - without any modification - and in a very broad concentration range. Due to the capillary format the method consumes only low sample material. NanoDSF allows us to study thermal stability (Tm) and aggregation onset temperature within one experiment. The high data point density enables to see even smallest transition points. Output data are highly comparable to DSC, but obtained faster, with less material and less costs. The Prometheus device is very robust and occupies only small lab space. We can definitely recommend this technology as nanoDSF has become a standard application in our lab."

Review date: 01 Dec 2016 | Prometheus NT.48 for protein stability
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Targenomix GmbH



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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It perfectly complements our workflow of protein biochemistry analytics.
Rating: 5.0

  • Application Area:Analyze enzyme stability and ligand binding

"The Prometheus solved a crucial step in our protein biochemistry workflow: we are able to quickly, efficiently and cost efficiently analyze the melting behavior of enzymes."

Review date: 30 Nov 2016 | Prometheus NT.48 for protein stability

The Prometheus NT.48 measures thermal unfolding and turbidity in parallel using near-UV detection systems. With nanoDSF and backreflection technologies, researchers can make better decisions about the stability of their protein formulations while using small volumes of sample. It is a great addition to any lab interested in small-scale thermal shift assays, profiling mutant protein behavior, or formulating storage and assay buffers.

Key Features:

  • Label-free measurements in solution
  • Broad concentration range, as low as 0.02 mg/ml to >200 mg/ml protein
  •  Measure intrinsic fluorescence of your proteins with dual UV system: 330 nm and 350 nm fluorescence detection
  • Monitor amorphous protein aggregates with near-UV turbidity detection
  • Capillaries hold 10 μl of sample; measure up to 48 samples at a time with single capillaries 
  • nanoDSF parameters: Tm, Tonset, reversibility of unfolding
  • Backreflection parameters: Tturbidity (onset of turbidity, particles >12.5nm in radius)
  • Temperature range: from 15 °C to 95 °C (up to 110 °C with High Temperature Upgrade)
  • Upgrade your instrument with software for chemical denaturation measurements (ΔG, c50) or complex thermal cycling ramps (isothermal, temperature cycling, stepped increments)
  • Maintenance-free instrument
  • CFR 21 Part 11 compatibility 
  • Straightforward handling: simple sample preparation and intuitive software user interface
     

Product Overview

Prometheus NT.48 for protein stability by NanoTemper Technologies product image

Prometheus NT.48 for protein stability

Manufacturer NanoTemper Technologies  |  Available Worldwide

4.9 / 5.0 | 7 reviews