Status:
ReviewerMember since: 2022
Organization: Institute of Molecular Biology
Oligo pool with excellent quality that I highly recommend
Application Area: Deep mutational scanning of protein degrons
"I ordered a 68bp oligo pool with >5000 sequences for deep mutational scanning of protein degrons. The pool had good yield and was easy to amplify. The pool was also very uniform. More than 96% of the desired sequences could be detected after all data analysis steps. Error rate is low. I could rarely see sequences that were not included in the original oligo pool design."
Status:
ReviewerMember since: 2021
Organization: California Institute of Technology
Good service with great product on oligopool synthesis.
Application Area:FISH
"Easy platform to use and order complex oligopool for downstream FISH probe generation. So far, every oligopool have been working and yield is reasonable. The fast turnaroud time is important for scientific work and it is also very easy to talk to their customer service support for anything regarding the order."
Status:
ReviewerMember since: 2021
Organization: DKFZ
Best in class low error rates and cheap pricing.
Application Area:Synthesis of barcoded DNA fragments for cloning
"TWIST's platform is super simple, their DNA synthesis tech has best-in-class error rates, and cheapest pricing. Turnaround time is sometimes a day or two slower than competitors, but as the error rate is so low we save time overall."
Status:
ReviewerMember since: 2020
Organization: UC Berkeley
Uniform oligo pool
Application Area:Library for peptide screening
"We ordered the 150mer oligo pool coding for a peptide library, to be used in a binding screen. The oligo pool was highly uniform, which turned out to be very valuable for our screening pipeline. We were able to avoid over and underrepresented peptides, and retrieve ~97% designed peptides in our screen."
Status:
ReviewerMember since: 2020
Organization: Stanford University
High quality, wouldn't have gotten my PhD without them!
Application Area:Test thousands of different genetic designs at once
"Twist Oligo Pools are a great way to test hundreds to thousands of different genetic designs at once. Great diversity and quality of oligos."
Oligo pools are utilized in many applications including generation of CRISPR guide RNA (sgRNA) libraries, peptide screens, protein screens, and high-throughput reporter assays. Accuracy and uniformity of oligo synthesis are critical to ensure high quality oligo libraries are built and screens are precisely targeted. Our industry-leading low synthesis error rate and uniform sequence representation result in high quality custom oligo libraries that enable the most efficient screens.
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