NucleoCounter® NC-202™ automated cell counter
The NucleoCounter® NC-202™ automated cell counter is our 3rd generation cassette-based image cytometer. Featuring improved hardware and new NC-View™ software, we take precision, speed and data management to new levels.
Receive your quote directly from the manufacturer.
High Recommend the NC-202
Cell Therapy
The NC-202 is an amazing piece of equipment, and ChemoMetec is a great company to work with. We were supported by Dr Anastasie Mate De Gerando, who is highly knowledgeable and always quick to respond to any questions we had.
Review Date: 13 Jan 2026 | ChemoMetec
User friendly, quick and efficient.
Bioprocess Internship
The NC202 was used extensively throughout our summer internship programme at Extracellular. This allowed bioprocessing interns to effectively monitor the growth and health of QM7 cells on a daily basis. This piece of equipment was excellent for users new to the field, providing a user friendly interface with efficient, quick and reliable data.
Review Date: 5 Jan 2026 | ChemoMetec
Excellent instrument: accurate, robust, and easy to use.
Engineered CHO cell culture characterisation
The instrument was used to monitor CHO cell cultures in an academic setting. The cells often expressed fluorescent proteins as part of our cell experimental workflow. We speculated that there was a possibility of interference between fluorescent protein expression (especially mOrange) and the viability measurements returned by the NC-202 Nucleocounter. We were very pleased to see that fluorescent protein expression had no impact on the accuracy of the viability measurements. Moreover, we had the opportunity to benchmark the NC-202 with manual counting and another automated cell counter from a different provider. The accuracy in quantifying cell density and viability of all our CHO cell cultures was comparable across all methods. Importantly, the ease of use of use of the NC-202 exceeded that of the other two methods, as it did not require dilution or further sample handling. Overall, we were very impressed by the NC-202's performance! I also want to mention that engagement from the ChemoMetec team (commercial and technical) was exceptional! Very rapid response times and genuine interest in assisting in my group's work. Thank you!
Review Date: 5 Nov 2025 | ChemoMetec
Reliable results, no matter the sample!
Vaccine research (immunology, target discovery, vaccine development)
At Infinitopes, precision really matters to us — not only in our target discovery but also in cell counting which is imperial for producing our vaccine vectors and their quality assessments, to testing how our epitopes trigger immune responses. Accurate ad consistent cell counts are key to keeping our research reliable and our data solid. We have used the NC-202 across a range of cell types — HEK293, A549, A375, CaSki, SHP77, and various primary cells — and it’s handled them all with no problems. For adherent cell lines, once they’re trypsinised, counting is straightforward. But even when we skip trypsinisation and leave cells in their natural, sometimes clumpy state, the NC-202 still delivers. Its lysing solution has been a game-changer for getting accurate counts where trypsinisation was not possible for our antigen discovery experiments or when manual methods were to laborious. Compared to the multiple other machines we’ve trialed, the NC-202 stand out first. Its software does an excellent job of recognising and counting cell clumps, and the lysing solution really solves any aggregation issues. We’ve also had great results with PBMCs, which are notoriously tricky due to their size and shape differences. The dedicated PBMC protocol has given us counts that match manual accuracy and since the system is 21 CFR Part 11 compliant it means that is suitable for our clinical trial work that is underway. Overall, the NC-202 has proven incredibly versatile and dependable. Whatever type of sample we’ve thrown at it, it’s delivered accurate, reproducible results - usually you can't have both and there's a trade-off in between the two, but not in this case.
Review Date: 3 Nov 2025 | ChemoMetec
Essential to daily operations
Bioprocess development - Microcarriers & Single cell suspensions
My team uses this instrument on a daily basis. We predominantly do upstream bio-process development for our own products as well as customer projects on viral vaccines, AAV, MSCS and iPSCs. Spinner flasks and stirred tank bioreactors provide a significant challenges in establishing a process, with a cornerstone in the cell biology and cell counting. The NC202 reduces the subjectivity experienced with other bench-top cell counting methods and instruments. After initial cell-specific protocol development, internal and external technology transfer has one less barrier. Every biotech and biopharma we work with, uses a NucleoCounter. Ease of use and training on this technology is another bonus. Biotechnology should take a lesson from the justice system: "Eye-witness testimony is the most flawed, but catching the events on a CCTV..." The above strongly offsets the cost and waste generation from the cartridges if there are high sample numbers to analyze.
Review Date: 8 Apr 2025 | ChemoMetec
Great product, great support
Enumeration or primary cells
Great device, it is fast, reliable and easy to use. The level of support ChemoMetec provide is second to none. Highly recommended.
Review Date: 24 Jan 2025 | ChemoMetec
Great product
Cell counting
Great product, great customer service!
Review Date: 23 Jul 2024 | ChemoMetec
Very good counting device, don't want to use another one anymore.
Cell counting human PBMCs and cell lines
Easy to use and fast. Highly reproducible results. Gives many parameters of counting results e.g. Total cell counts, viable cells, dead cells, vitality of cultured cells which are very helpful for documentation.
Review Date: 6 May 2024 | ChemoMetec
Great system, we use it daily and recommend to all of our clients
Cell therapy
The NC202 has simplified our cell count and viability processing, enabling smooth comparison between process development and GMP. The Chemometec team have been very supportive throughout our development and transition between the NC200, 250 and 202.
Review Date: 2 May 2024 | ChemoMetec
Great results, highly recommended
Cell counting
Equipment of high effectiveness and great customer support. We obtain high quality and reproducible results, very easy to use.
Review Date: 26 Apr 2024 | ChemoMetec
The NucleoCounter® NC-202™ automated cell counter is our 3rd generation cassette-based image cytometer. Featuring improved hardware and new NC-View™ software, we take precision, speed and data management to new levels. The NucleoCounter® NC-202™ is the most precise automated cell counter on the market: The combined inter-operator/inter-instrument variation is typically 5 – 10%. Consequently, you obtain identical results with any cell sample, recorded by any user, located anywhere in the world.
The NucleoCounter® NC-202™ has one universal cell counting protocol that handles all cell types: No optimization is required. The instrument provides robustness and precision regardless of cell size, morphology and media type, even in the presence of red blood cells, magnetic beads or cellular debris. Consequently, the NucleoCounter® NC-202™ is easy to validate, even for the most complex processes.
The all new DebrisIndex™ is a measurement of culture debris build-up. Debris negatively affects downstream filtering and purification steps, so a direct measurement of debris facilitates better process development parameters. The NC-View™ software is compatible with 21 CFR Part 11/GMP guidelines and can be integrated into your clinical workflow.
Our Field Application Scientists (FASs) provide training, service visits, installation and user guidance on-site or online to ensure you get the best start to working with the NucleoCounter® NC-202™.
What’s NEW – NucleoCounter® NC-202™:
- Automated cell counter
- Analysis time only 30 seconds
- Improved light sources for greater signal-to-noise ratio and dynamic range
- DebrisIndex™ monitors cell culture health and bioreactor production runs
- Broader linearity range: Count samples up to 1×107 cells/ml
- Instrument Service Plan: Keep your instrument up-to-date
- Automatic data quality checks for sample artefacts
- Electromechanical cassette slot for easy operation
Counting embryonic and induced pluripotent stem cells on route to treating diabetes
In this whitepaper, ChemoMetec demonstrates how the technology of the NucleoCounter® NC-202™ automated cell counter can be used to count and monitor cells during β-cell generation accurately and precisely. Additionally, showing that the aggregated cell count assay enables a robust and precise solution to the challenge of counting highly aggregated β-cell clusters.
Standardizing the cell count in CAR T cell therapy
Considering the competitive clinical landscape of CAR-cell therapies, accelerated development timelines and the variable nature of cellular therapies, designing a robust process should begin early to make transitioning to the clinic and scaling upwards or outwards smoother.
In this eBook, ChemoMetec explains the fundamentals of accurate and precise cell counting in that process, highlighting the unique features of its flagship cell counter, the NucleoCounter® NC-202™.
NucleoCounter: Publications review
In 2024, ChemoMetec was featured in over 150 publications spanning various research areas, including cell biology, neurology, gene therapy, oncology, stem cells, bioprocessing, and cell-based immunotherapy.
In this eBook, ChemoMetec presents 30 selected publications that show case the versatile usage of its NucleoCounter® NC-200™, NC-202™, and NC-3000™ instruments across multiple disciplines.
Manual vs. automated cell counting: Overcoming four major sources of error in manual cell counting
In this application note, Chemometec explains how to overcome the four main challenges in cell counting: human perception of what defines a cell; volume, dilution, and pipetting errors; viability determination; counting enough events.
NucleoCounter® NC-202™: Performance Data
The NucleoCounter® NC-202™ is a high precision cell counter using low magnification fluorescence microscopy and automated image analysis to identify live and dead cells. This application note demonstrates the performance of the NucleoCounter® NC-202™ in comparison with manual cell counting.
NucleoCounter® NC-202™: Cell Count & Viability of Yeast Cells using Via2-Cassette™
The NucleoCounter® NC-202™ automated cell counter and NC-View™ software perform cell counting and viability analyses on a broad range of eukaryotic cells. The Via2-Cassette™ provides a simple and robust method to determine cell concentration and viability with the NucleoCounter® NC-202™. This application note demonstrates the cell concentration and viability of Saccharomyces and Schizosaccharomyces yeast cells using the Via2-Cassette™ and Reagent Y200 (yeast permeabilization buffer).
NucleoCounter® NC-202™: Cell Count & viability using Via2-Cassette
The NucleoCounter® NC-202™ automated cell counter and NC-View™ software perform cell counting and viability analyses on a broad range of eukaryotic cells. The Via2- Cassette™ provides a simple and robust method to determine cell concentration and viability with the NucleoCounter® NC-202™. This application note demonstrates the cell concentration and viability of mammalian cells using the Via2-Cassette™.
NucleoCounter® NC-202™: Cell Count & Viability of Cells Grown on Microcarriers using Via2-Cassette™
The NucleoCounter® NC-202™ automated cell counter and NC-View™ software perform cell counting and viability on a broad range of eukaryotic cells. This application note demonstrates the cell concentration and viability of a variety of cells grown on microcarriers using the Via2- Cassette™ and Lysis 1 with the NucleoCounter® NC-202™.
NucleoCounter® NC-202™: Cell Count & Viability of Insect Cells using Via2-Cassette™
The NucleoCounter® NC-202™ automated cell counter and NC-View™ software perform cell counting and viability analyses on a broad range of eukaryotic cells. The Via2-Cassette™ provides a simple and robust method to determine cell concentration and viability with the NucleoCounter® NC-202™. This application note demonstrates the viability and cell concentration of insect cell lines (e.g., S2, Sf9, Sf21 etc.) using the Via2-Cassette™.
NucleoCounter® NC-202™: Cell Count & Viability of PBMCs using Via2-Cassette™
The NucleoCounter® NC-202™ automated cell counter and NC-View™ software perform cell counting and viability analyses on a broad range of eukaryotic cells. The Via2-Cassette™ provides a simple and robust method to determine cell concentration and viability with the NucleoCounter® NC-202™. This application note demonstrates the viability and cell concentration of peripheral blood mononuclear cells (PBMCs) using the Via2-Cassette™.
NucleoCounter NC-202
The NucleoCounter® NC-202™ automated cell counter is ChemoMetec's 3rd generation cassette-based instrument. Featuring improved hardware and the new user-friendly NC-View™ software, it takes precision, speed, and data management of cell counting to new levels.
Discover the Via2-Cassette: Eliminate human error for a robust cell counting method
ChemoMetec, a leading manufacturer of cell counting and analysis equipment, demonstrates how its Via2-Cassette™ device can give precise, accurate, and reproducible cell count and viability analysis in just 30 seconds - whilst eliminating the potential for human error.
Scaling your cell culture system: Optimizing bioreactor conditions
In this video by Chemometec, learn about culturing conditions in bioreactors for the following applications: stem cells, monoclonal antibodies and vaccine development. Also, learn about the pros and cons of different bioreactor systems and the best practices for monitoring the health and growth of your cell culture system through automated cell counting.
Podcast: Solving the challenges of standardizing cell counting to ensure reproducibility in experiments, assays and manufacturing processes
In this podcast, Christian Berg, Global Product Manager at Chemometec talks about the importance of standardizing cell counting because while often overlooked, it is essential for reproducibility in experiments, assays, and manufacturing processes. Cell counting is a challenging technique, with many pitfalls, that can delay entire projects. This podcast discusses how new technologies are solving these challenges and enabling standardization of cell counting across organizations.
ChemoMetec achieves gold status with a new Seal of Quality award
ChemoMetec's family of cell counters are being recognized for receiving consistently outstanding customer feedback, again
ChemoMetec achieves silver status, with a new Seal of Quality award
These cell counters are being recognized for receiving consistently outstanding customer feedback, again
Consistent cell count & viability for development & manufacturing
ChemoMetec is determining viability and cell count precisely with its new technology
