MOBIE

In this application note, MOBILion Systems, Inc. delves into the challenges of developing therapeutic agents from natural extracts. The complexity of these extracts, with their diverse chemical compositions, poses significant hurdles in identifying bioactive compounds. The presence of isomers, molecules with identical formulas but differing spatial arrangements, adds further complexity. The note showcases the utility of high-resolution ion mobility (HRIM) by analyzing 28 cannabinoids from cannabis sativa. The application of HRIM provides advantages like separating isobaric and isomeric compounds, potentially increasing sensitivity by reducing background noise, and adding collision cross section as a complementary identifier to mass spectra. The study employed a simple and rapid liquid chromatography method utilizing Agilent 1290 LC, an integrated MOBILion Systems MOBIE® instrument, and an Agilent 6546 Q-TOF for the analysis of 28 cannabinoids, including 15 isomers.

In this application note, MOBILion Systems, Inc. explores the challenges posed by per- and polyfluoroalkyl substances (PFAS), known as 'forever chemicals,' due to their elusive nature and limited detection methods. The concept of PFAS 'dark matter' refers to compounds undetectable by conventional techniques, leading to gaps in our understanding. The note introduces forensic fingerprint analysis as a promising tool, utilizing high-resolution ion mobility (HRIM) coupled with high-resolution mass spectrometry (HRMS) to shed light on PFAS distribution, impact, and mitigation. By integrating HRIM with existing liquid chromatography methods, this approach can identify branched isomers and provide a more comprehensive understanding of these persistent compounds. The study successfully resolved 11 isomers, including previously unresolvable compounds, highlighting the potential of HRIM in enhancing PFAS analysis.

In lipidomics, the challenge is accurately identifying isobaric and isomeric species in lipidomes. This shift from untargeted to targeted lipid analysis demands advanced solutions due to complex structures. Current methods struggle to resolve these issues. This application note shows how the MOBIE® platform provides a uniquely flexible platform that can be tailored to suit different lipid workflow solutions.

In this application note, MOBILion Systems demonstrates the benefit of incorporating high-resolution ion mobility (HRIM) into traditional LC-MS workflows for reproducible high-throughput targeted post-translational modifications (PTM) analysis for biopharmaceutical characterization.

Due to growing concern over their toxicological significance, analytical methods for PFAS (Per- and Polyfluoroalkyl Substance) are continuing to evolve. Targeted methods have historically been utilized with liquid chromatography and tandem mass spectrometry (MS/MS) the predominate combination used. In this application note, MOBILion Systems demonstrates the combination of liquid chromatography (LC), high-resolution ion mobility (HRIM), and high-resolution mass spectrometry (HRMS) for PFAS analysis.

Download this white paper, to see a demonstration of the capabilities and utilization of standard method templates for high throughput flow injection analysis coupled with high-resolution ion mobility-mass spectrometry (HRIM-MS) for positive-mode lipid analyses.

In this application note, explore how MOBILion’s HRIM-MS platform can be used as a high throughput alternative to long LC-MS gradients by providing a rapid peptide mapping protocol.

Incorporating HRIM into an LC-MS workflow provided >96.5% sequence coverage in just 20 minutes total analysis time and included critical structural information that would have been missed using conventional LC-MS techniques alone.

Catch up on some of the most exciting advancements in mass spectrometry presented at ASMS 2022

Scientific, technological, and communications excellence has been celebrated at SLAS2022 with MOBILion Systems, Agilent Technologies, and Wyatt Technology Corp. among those recognized