Mix & Go! E. coli Transformation Kit by Zymo Research

Manufacturer Zymo Research  |   Model: T3001
4.9
/
5.0
  |  8 reviews
The Mix & Go E. coli Transformation Kit & Buffer Set allow you to make your own highly efficient chemically competent cells. Simply add DNA and spread onto a plate - Mix and Go!


Mix & Go! E. coli Transformation Kit by Zymo Research product image
Mix & Go E. coli Transformation Kit Buffer Set
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Average Rating: 4.9
8 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Advanced Reviewer
  • Member since: 2018

  • Organization: Broad Institute



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Easy and reproducible to prepare high-titer competent cells.
Rating: 5.0

  • Application Area: Prepare competent cells

"We regularly use the product for the preparation of competent E.coli. The procedure simple; wash the log-phase E.coli culture with the wash buffer in the kit and then re-suspend it in the competent buffer – that's it! You don't need worry about a complex buffer, multiple washing, and a unreproducible titer!"

Review date: 29 Apr 2020 | Mix & Go! E. coli Transformation Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Pennstate College of Medicine, Hershey



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Excellent product for a great price!
Rating: 4.7

  • Application Area:Preparation of competent cells for molecular biology applications

"In molecular biology, bacterial transformation is a basic and essential step. Traditional methods of E. coli competent cell preparation take a lot of time and effort. This particular product from Zymo offers a simple method of competent cell preparation. This kit is very robust and the method is very simple. For the competent cells prepared by this method, heat shock is not required for the transformation. For transformation: thaw E. coli on ice and add required amount of DNA (1-5 ul) per 50 ul cells. Keep on ice for 5 minutes. Spread on a pre-warmed LB plate and incubate overnight at 37 deg C. The efficiency is near>10^7/µg (number of colonies observed after transformation). This kit is budget-friendly and at the same time, there is no compromize with the quality. Overall it's an efficient kit and highly recommended."

Review date: 27 Nov 2019 | Mix & Go! E. coli Transformation Kit
  • Status:

    Advanced Reviewer

  • Member since: 2019

  • Organization: university of florence, Italy



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Making ultracompetant bacterial cells just got easy!
Rating: 4.7

  • Application Area:Prepare ultracompetant cells

"In our lab we do an average of 25 transformations per week, considering the high usage we prefer to prepare make our own cells. We always used the standard protocol to make the buffer which is always a pain especially as it just tends to be more laborious with the preparation of all the buffers. Here we just grow our bacteria at 18C untill we reach the OD of 0.5 and then boom, just two simple steps with washing and resuspension and then the cells are ready. We did the same for DH5alpha and STBL3 strain and got competancy in the range of 10^8. It's pretty good and it isn't that expensive. P.S: We didnt bother using the Zymobroth, we used our regular SOB. But it works well!"

Review date: 27 Nov 2019 | Mix & Go! E. coli Transformation Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: tulane university



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Good kit, fast.
Rating: 5.0

  • Application Area:Plasmid construction

"Very good, not expensive, easy to use, every lab can try this. Much cheaper than that of other companies, very fast, only takes 10 min."

Review date: 05 Jun 2019 | Mix & Go! E. coli Transformation Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Texas A&M University



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Excellent efficiency with DH5alpha.
Rating: 4.7

  • Application Area:Transformation of vectors in E. coli

"Pretty good experience with the transformation kit. Usually we use electroporation for transformation. This kit takes about a 3rd of the time and the transformation efficiency is as good/better. Works like a charm with DH5alpha and circular DNA (plasmids), not sure about linear DNA/other strains: I tried it once, got a mix of colonies: carrying/not carrying the linear DNA, but that might have been a problem with the strain too."

Review date: 05 Jun 2019 | Mix & Go! E. coli Transformation Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Research Institute



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Making E.coli competent is not difficult, but this kit makes it reproducibly trivial.
Rating: 5.0

  • Application Area:Making competent cells

"Making E.coli competent is not difficult, but this kit makes it truly trivial. Using standard DH10B, SOB and SOC media rather than the specialized media from Zymo, the efficiency is routinely >10^7/µg. No need for a heat shock. Depending on what I am transforming, the transformation reaction is scaled down to 25µl (standard restriction digest ligations, golden gate assembly and ligation independent cloning), or 5µl for supercoiled plasmid, making the kit even more economical. By including a recovery step, transformation with Kan resistant plasmids is as efficient as Amp resistant."

Review date: 28 Jul 2016 | Mix & Go! E. coli Transformation Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Rowan SOM



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Excellent product for labs doing a lot of cloning.
Rating: 5.0

  • Application Area:Cloning & Transformation

"Good price compared to store bought competent cells, very easy and quick to use, excellent alternative to normal cloning & transformation especially when time needed for normal incubations associated with transformations isn't available."

Review date: 27 Jul 2016 | Mix & Go! E. coli Transformation Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Texas



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results. Easy to use.
Rating: 5.0

  • Application Area:E.coli transformation for cloning

"Easy to prepare the competent cells and the transformation efficiency is great."

Review date: 21 Jul 2016 | Mix & Go! E. coli Transformation Kit

The Mix & Go E. coli Transformation Kit and Mix & Go E. coli Transformation Buffer Set are convenient methods for the preparation of competent E. coli cells for simple and highly efficient DNA transformation. The Mix & Go method completely eliminates the requirement for heat shocking and related procedures. Instead, Mix & Go bacterial transformation can be performed by adding DNA to Mix & Go competent cells and spreading onto a plate. Transformation efficiencies are typically on the order of 10 8 -10 9 transformants/µg plasmid DNA with most E. coli strains.

Uniquely formulated reagents make it easy to generate Mix & Go competent cells from current E. coli strains that are available in the laboratory. Simply grow the E. coli strain of your choice, wash, then resuspend the cells in the provided buffers. The cells are now transformation ready! The Mix & Go E. coli Transformation Kit includes all buffers and ZymoBroth™ medium to generate 20 ml of Mix & Go competent cells. The Mix & Go E. coli Transformation Buffer Set includes all buffers that are required to generate 60 ml of Mix & Go competent cells, and the medium (broth) is supplied by the user.