Member since: 2019
Organization: Concept Life Sciences
Excellent upgrade of both software and hardware. Maintenance is so much easier!
Application Area: Drug discovery
"The MicroCal PEAQ-ITC is a significant upgrade from the VP-ITC and ITC200 in terms of the ease of maintenance. All washing steps are now automated and easy to perform. We find this helps to keep the instrument in an excellent condition even with multiple users. Due to the somewhat complicated cleaning procedures of previous versions of the ITC we found that routine maintenance was not always performed which greatly affected results in this highly sensitive instrument. The PEAQ is still a low volume isothermal titration calorimeter ideal for label-free, in-solution studies of biomolecular interactions. Software upgrades have also made the PEAQ easy to use even for the most inexperienced scientists in the lab, including help in experimental planning and optimisation of parameters prior to performing any runs. We find the PEAQ really useful for confirming binding, measuring stoichiometry, protein QC, troubleshooting other biophysical methods and identifying different mechanisms of binding in closely related compounds."
Member since: 2019
Organization: Boise State University
Application Area:Protein/protein interactions; protein/small molecule interactions; protein/nucleic acid interactions
"Excellent ease-of-use. Video training modules allow for users across disciplines to rapidly collect data."
Manufacturer's ResponseThank you Lisa for your review. We are very happy to hear that you like the ‘in-built’ videos. We have had lots of feedback that we shouldn’t expect all our users to be experts or to use the instruments frequently. We have hoped that these videos would allow people to walk up to the instrument and use it straight away without much training.
Member since: 2016
Organization: California Lutheran University
This is a bread and butter instrument for our lab.
Application Area:Protein-substrate binding and kinetics
"There is a higher upfront cost than with other ITCs, but the available nascent kinetics parameters and lower cost consumables make it a good investment in the longer term."
Manufacturer's ResponseThank you very much for your feedback.
The MicroCal PEAQ-ITC is a highly sensitive, low volume isothermal titration calorimeter for the label-free in solution study of biomolecular interactions. It delivers direct measurement of all binding parameters in a single experiment and can analyze weak to high affinity binders, using as little as 10µg sample. Semi-automated maintenance minimizes operator intervention and the system is upgradable to the fully automated MicroCal Auto-iTC200, making it ideal for laboratories where speed, sensitivity and the ability to accommodate higher workloads in the future are paramount.