Member since: 2005
Organization: Università degli Studi di Padova
Great data, fast to be obtained, easily interpretable! Useful for small molecules!
Application Area: Analyzed lead compounds (small molecules) that inhibit an enzyme
"ITC is a friendly technique, and this instrument has been upgraded, making it quite robust and easy to use. For biochemists, PhD students as well as PostDocs, it represents an excellent source of info for studying protein-small molecules interactions. If your molecules are water soluble or solubility can be achieved by adding a small amount of organic solvent to your buffer solutions, it is the method of choice to obtain thermodynamic parameters for your lead molecules, affinity constant, enthalpy, stoichiometry of interaction and so on... Last but not least, it helps a lot when you want to know if any compounds do their job by interacting directly with your target or not."
Manufacturer's ResponseThank you Professor Cendron. It is very kind of you to write this. As part of the team that developed the PEAQ-ITC, it is very gratifying to hear that we have been successful, at least in part, at making the instruments user friendly and robust.
Member since: 2020
Organization: Syracuse University
Great instrument, good service.
Application Area:Protein metal binding
"I like the instrument design. It is easy to operate, all parts seem very sturdy and there are no extra elements. Malvern did a good job writing the manual for this instrument which is helpful for troubleshooting your experiment and also for maintenance. The software is nice and user-friendly, has a lot of functions that can help you analyze your experiment in the best way. The only issue is that the equations used for calculations are not provided in the software, they are discussed in the manual. As a visual person, I would prefer seeing all calculations but that's just me. After installation, we had a few training sessions (one with the service engineer and another with the application specialist) which were definitely enough to start using the instrument with confidence. As for the maintenance, we recently had our annual check and everything was fine. The engineer came for a day, tested and cleaned our instrument. The only issue was the communication with the service engineer as he was not the best in responding and it took some time to schedule his visit. Many thanks to Stoyan who always answers all questions about experiment design and data analyses. His experience definitely helps in troubleshooting."
Manufacturer's ResponseThank you. Very nice to hear that you like the instrument. Thank you also for the feedback on the timeliness of our response to scheduling a visit. We have fed that back to the team. I encourage you to reach out to your local representative who will be happy to assist you and ensure that doesn’t happen again.
Member since: 2020
Organization: Lodz University of Technology
I love this instrument!
Application Area:Analysis of the affinity of enzymes for polyphenols
"The device is easy to use. The small amount of substance to be analyzed is a big advantage. This is a great saving of money. It is a precision instrument with high sensitivity and accuracy. In one analysis we get all the thermodynamic parameters needed to develop inhibitors."
Manufacturer's ResponseThank you Joanna, very interesting. It is very rewarding to hear that the effort that we put into the design and usability of the instrument has benefitted you and, indeed, saved you money. It would be wonderful if you could share the savings that this instrument has brought to you versus the previous models
Member since: 2020
Organization: Dartmouth College
Mediocre instrument with very few advantages over the VP-ITC.
Application Area:Metal binding to metalloproteins
"Although I appreciate that the low-volume Peaq-ITC allows for rapid testing (~8 experiments per day on average) the overall use of the instrument is miserable. It will randomly disconnect from the computer every once in a while. Luckily this hasn't happened during an experiment, but is frustrating when you go to start an experiment and you have to shut down the computer to get the instrument to reconnect again. Furthermore, although the automatic cleaning is useful, it is the most frustrating aspect of the instrument. Too many parts and seals necessary. Most ridiculous is that you have to wait 7-9 minutes before the cleaning system will tell you there is an error with the cleaning, and will cancel. So you check the connections and restart, wait 7-9 minutes again for it to fail again. You then get tired of waiting for it to clean and do a ridiculous manual clean (there is no good way to do a manual clean), and there will be an error stating the instrument hasn't been cleaned in XX-uses. At the minimum there should be an override control of the cleaning system so experiments can continue. To top it off, the most frequent problem that causes this cleaning issue is part #: ASY5020090. A little tube with a connector and a little black seal for ~$300, which we have to replace every 3-4 months, as that is approximately how long we have until this little part fails and we can no longer utilize the instrument because it will not clean. The sensitivity is high on the instrument, which is good. However, when systems has low heat, the signal-to-noise ratio is very high and the baseline is terrible. This, in turn, makes you use more sample at a higher concentration, wholly defeating the purpose of this instrument. The error between experiments is about 2-3 times higher than the error between experiments on the VP-ITC as well, which is ridiculous. I need to do ~6-8 experiments to obtain the same error on 3 experiments by the VP-ITC. So basically you can do quicker experiments, but you need the same overall amount of time to get reasonable data. Lastly, the data analysis software is of poor quality. The switch to proprietary data analysis has made data analysis cumbersome, compared to the use of Origin as the fitting software. Two-Site fitting is backwards when binding site #1 is actually binding site #2. The fitting curve gets stuck when trying to fit two-site data, and requires extensive manual fitting to get it to work, with questionable results and thermodynamics. Although this is a small complaint, when making the isotherms publication quality, you have to use individual drop-downs for every little piece of the graph. The lines are not even black as the default, but a weird blue-grey color. Changing the raw data to black then the axis labels to black, then switching and changing the fitted data colors takes a ridiculous amount of time. Plus there appears to be ~100 color choices, which is just overkill."
Manufacturer's ResponseWe thank you very much for taking the time for providing this feedback. We regret very much that you are less than happy with the instrument. Please be assured that we take this very seriously and will include your comments in our new design discussions for product improvements. In the meantime please feel free to contact your local representative if there is anything we can do to help you in the short term.
Member since: 2019
Organization: University of Macau
Generally, it's good.
Application Area:Supramolecular chemistry
"A very good instrument, however, the small volume of cell and syringe cannot meet the requirements of supramolecular chemistry well. Generally, the small volume can save reagents, but for some specific cases, it limits the application of PEAQ-ITC."
Manufacturer's ResponseThank you very much for the feedback? It is great to hear that you find the instrument very good, easy to use and that the small volume can save on reagents. We’d really like to hear about the supramolecular chemistry applications that we could better support with future product developments so please me at firstname.lastname@example.org
Member since: 2019
Organization: Concept Life Sciences
Excellent upgrade of both software and hardware. Maintenance is so much easier!
Application Area:Drug discovery
"The MicroCal PEAQ-ITC is a significant upgrade from the VP-ITC and ITC200 in terms of the ease of maintenance. All washing steps are now automated and easy to perform. We find this helps to keep the instrument in an excellent condition even with multiple users. Due to the somewhat complicated cleaning procedures of previous versions of the ITC we found that routine maintenance was not always performed which greatly affected results in this highly sensitive instrument. The PEAQ is still a low volume isothermal titration calorimeter ideal for label-free, in-solution studies of biomolecular interactions. Software upgrades have also made the PEAQ easy to use even for the most inexperienced scientists in the lab, including help in experimental planning and optimisation of parameters prior to performing any runs. We find the PEAQ really useful for confirming binding, measuring stoichiometry, protein QC, troubleshooting other biophysical methods and identifying different mechanisms of binding in closely related compounds."
Member since: 2019
Organization: Boise State University
Application Area:Protein/protein interactions; protein/small molecule interactions; protein/nucleic acid interactions
"Excellent ease-of-use. Video training modules allow for users across disciplines to rapidly collect data."
Manufacturer's ResponseThank you Lisa for your review. We are very happy to hear that you like the ‘in-built’ videos. We have had lots of feedback that we shouldn’t expect all our users to be experts or to use the instruments frequently. We have hoped that these videos would allow people to walk up to the instrument and use it straight away without much training.
Member since: 2016
Organization: California Lutheran University
This is a bread and butter instrument for our lab.
Application Area:Protein-substrate binding and kinetics
"There is a higher upfront cost than with other ITCs, but the available nascent kinetics parameters and lower cost consumables make it a good investment in the longer term."
Manufacturer's ResponseThank you very much for your feedback.
The MicroCal PEAQ-ITC is a highly sensitive, low volume isothermal titration calorimeter for the label-free in solution study of biomolecular interactions. It delivers direct measurement of all binding parameters in a single experiment and can analyze weak to high affinity binders, using as little as 10µg sample. Semi-automated maintenance minimizes operator intervention and the system is upgradable to the fully automated MicroCal Auto-iTC200, making it ideal for laboratories where speed, sensitivity and the ability to accommodate higher workloads in the future are paramount.