LUNA-FL™ Dual Fluorescence Cell Counter

In this application note, Logos Biosystems compares the cell counting results of the LUNA™ Reusable Slide, a hemocytometer, and disposable slides.

This white paper addresses areas of florescence cell counting including fluorescence dyes, applications and a comparison of technologies. The LUNA-FL™ fluorescence cell counter is presented as a solution to overcome limitations associated with cell types.

This application note analyses the viability of Saccharomyces Cerevisiae stained with Fluorescein diacetate (FDA), a fluorogenic cell viability probe, using the LUNA-FL™, fluorescence cell counter.

An easy and popular method to measure the transfection efficiency is to use a GFP gene. For example, by making a fusion protein between GFP and the protein of interest, the expression of the protein of interest can be measured by the presence of GFP protein. This application note demonstrates that the Luna-FL™, is suitable for the measurement of GFP transfection efficiency.

June 12, 2025 – 15:00 BST / 16:00 CEST / 10:00 EDT / 07:00 PDT

As single-cell and multiomics technologies continue to reshape our understanding of complex biology, the importance of high-quality sample preparation has never been greater. Reliable quality control (QC) is essential to ensure that valuable samples - often limited and difficult to obtain - yield meaningful, reproducible data. However, researchers working with diverse sample types, such as frozen or FFPE tissue, often face significant QC challenges.

In this webinar, experts from both academia and industry share real-world approaches to QC in single-cell and nucleus workflows, a critical step for ensuring the success of downstream applications such as single-cell RNA sequencing (RNA-seq) and multiomics analysis.

The expert speakers will show how the LUNA-FX7™ automated cell counter enables accurate and reproducible QC, even in challenging samples with high debris levels or low yield. Through advanced imaging and algorithm-based classification, you will see how the FX7 supports reliable assessment of nuclei and cell concentration and viability – with data from a range of sample types including fresh, frozen, fixed, and FFPE preparations.

Key learning objectives:

• Explore real-world applications for improving sample quality in single-cell and nuclei workflows.
• Gain insights into accurate assessment of sample concentration and viability using the LUNA-FX7™ automated cell counter.
• Learn practical approaches for handling challenging samples such as fixed and FFPE preparations.

Who should attend?

From students and professors to lab technicians, managers, and chief scientific officers, covering academia, government, and private sector businesses and industries.

Certificate of attendance

All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes. If you view the on-demand webinar, you can request a certificate of attendance by emailing editor@selectscience.net.

To achieve desired and reliable outcomes in single cell sequencing, the accurate evaluation of nuclei quality is critical. Choosing the most effective method and choosing the right combination of dyes is not easy.

In this webinar, you will learn how to streamline nuclei assessments and choose optimal fluorescent dyes combinations. In addition, you will discover how to integrate the different dyes and optimize advanced automated cell counters to ensure the reliability of results.

Key learning objectives

• Learn how to streamline nuclei quality assessments for downstream applications such as single cell genomics research
• Find out the optimal fluorescent dye combinations for ensuring the quality and reliability of nuclei assessments
• Understand the capabilities of automated cell counters (like that of the LUNA-FX7™ and LUNA-FL™) as powerful tools for precise nuclei assessments

Who should attend?

Students, graduate students, doctoral students, post doc researchers, assistant/associate professors, professors, lab technicians, lab specialists, scientists, researchers, lab managers, lab supervisor/directors, principal investigators, principal/associate scientists, QA/QC manager/supervisor, chief scientific officer, VP of research and development (or any decision-making role). Covering both academia, government, and private sector industries.

Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

Cell counting remains an essential process across fundamental research, clinical applications and bioproduction.

As science continues to advance, there is a growing demand for faster, higher-throughput, and more tailored cell counting solutions.
While automated techniques address many of these demands, challenges including sample prep, technology compatibility, cost efficiency, and compliance remain.

Recent innovations in software and hardware, including the integration of artificial intelligence and machine learning, focus on enhancing accuracy and efficiency in cell counting.

Karine Labour, Europe Managing Director for Logos Biosystems, will explain how current and developing techniques support researchers reaching the critical milestone of data standardization, accuracy, and reproducibility in cell counting.

Key learning objectives

• Learn about current cell counting challenges, practical solutions using LUNA™ counters, and applications including CAR-T cell therapy production and single cell sequencing
• Understand how the LUNA-FX7™ achieves increased accuracy with higher throughput options, offering added flexibility and increased productivity
• Learn how the new LUNA-III™ cell counter uses machine learning algorithms to ensure high quality and reliable results

Who should attend?

• Students, graduate students, doctoral students, post doc researchers, assistant/associate professors, professors, lab techs, lab specialists, scientists, researchers, lab managers, lab supervisors/directors, principal investigators, principal/associate scientists, QA/QC managers/supervisors, chief scientific officers, VP of research and development (or any decision-making role), and those in both academia, government, and private sector industries.

Certificate of attendance
All webinar participants can request a certificate of attendance, including a learning outcomes summary, for continuing education purposes.

March 11, 2026 – 15:00 GMT / 16:00 CET / 11:00 ET / 08:00 PT

As single-cell and multiomics technologies continue to reshape our understanding of complex biology, the importance of high-quality sample preparation has never been greater. Reliable quality control (QC) is essential to ensure that valuable samples - often limited and difficult to obtain - yield meaningful, reproducible data. However, researchers working with diverse sample types, such as frozen or FFPE tissue, often face significant QC challenges.

In this SelectScience webinar, experts from both academia and industry share applications and methods to achieve quality control in single-cell and nucleus workflows to ensure the success of downstream applications. This is a critical step for applications such as single-cell RNA sequencing and multiomics analysis.

Using the LUNA-FX7™ automated cell counter, speakers will discuss how to achieve accurate and reproducible samples and master quality control, even with challenging samples containing high debris levels or low yields and share data from a range of sample types including fresh, frozen, fixed and FFPE preparations. In addition, you’ll learn how advanced imaging and algorithm-based classification, like that offered with the LUNA-FX7 supports reliable assessments of nuclei and cell concentration as well as cell viability.

Key learning objectives:

• Explore real-lab applications in both academia and industry to improve sample quality in single-cell and nuclei workflows.
• Gain insights into accurate assessment of sample concentration and viability using the LUNA-FX7™ automated cell counter.
• Learn practical approaches for handling challenging samples such as fixed and FFPE preparations.

Who should attend?

• Students, graduate students, doctoral students, post-doc researchers
• Assistant professors, associate professors, professors
• Lab technicians, lab specialists, scientists, researchers, lab managers, lab supervisors, lab directors, principal investigators, principal/associate scientists
• QA/QC managers and supervisors
• Chief scientific officers (CSOs), VPs of research and development, or any decision-making role covering academia, government, and private sector businesses and industries.

Certificate of attendance

If you attend the live webinar, you will automatically receive a certificate of attendance, including a learning outcomes summary, for continuing education purposes. If you view the on-demand webinar, you can request a certificate of attendance by emailing editor@selectscience.net.

Webinar details

• Cost: Free to attend
• Location: Online
• Duration: 60 minutes

Registration is required to secure your place. If you register but can’t attend live, you will receive a link to the on‑demand recording once it becomes available.

This video introduces the Luna FL™ Dual Fluorescence Cell Counter. The deual fluroescence allows for senstive analysis of hard to distinguish cell types, including blood cells.

Watch this video to learn about the Luna-FL™ Dual Fluorescence Cell Counter, and automated cell counter, integrating bright field and dual fluorescence optics together. Discover how the system provides sensitive and accurate live and dead cell counting results, without limitation of cell type.

This video demonstration introduces the features of the Luna FL Automated Cell Counter from Logos Biosystems. Learn how the system incorporates red and green fluorescence, as well as bright field microscope optics, and is suitable for a range of cell types including blood cells, primary cells, yeast, and standard cell lines.

Discover how the latest automated cell counters are critical to successful single cell genomics workflows

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