Member since: 2021
Organization: Fusion Genomics
Application Area: Synthesis of biotinylated probes
"Lambda exonuclease is renowned for its simplicity in synthesizing single-stranded DNA. I am using the enzyme to synthesize biotinylated probes for a targeted-capturing assay. While this synthesis method has the minimum layer of complexity, it failed to digest all the DNA input. According to the manual, 5U of Lambda exonuclease can digest 5µg of dsDNA. However, when I used 5U of lambda exonuclease to digest 2.5µg of dsDNA, the amount of ssDNA obtained was 75% of the initial input. Aside from that, lambda exonuclease is user-friendly and easy to use."
Lambda Exonuclease is ideal for:
Manufacturer New England Biolabs Inc. | Available Worldwide4.7 / 5.0 | 1 reviews