Invitrogen™ Alexa Fluor™ 647 Tyramide Reagent by Thermo Fisher Scientific

Alexa Fluor 647 Tyramide Reagent is a component of SuperBoost™ tyramide signal amplification kits now available separately. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC), and in situ hybridization (ISH).

SuperBoost reagents, when used as directed, can increase sensitivity up to 200 times over standard imaging methods. SuperBoost reagents are simple to use and easily adapted to standard ICC, IHC, or FISH experimental protocols, using any cell or tissue type. Alexa Fluor 647 Tyramide Reagent (650/668 ex/em) can be detected using standard Deep Red/Cy5 filter cube.

Features: of the SuperBoost reagents include:

• Superior sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
• Simple protocol and detection using standard filters
• Suitable for high-resolution multiplex images—co-label with TO-PRO™-3, secondary antibodies, and other SuperBoost kits
• Requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments