The Human T Cell Memory Cell and Cytokine Profiling Kit measures T cell memory phenotype and function at different stages while providing information about their health and their role in cytokine secretion.
This one wash assay requires minimal hands on time and measures both cells and beads to provide the following biological insights: Live immune cells are distinguished from dead cells by staining with a fluorescent membrane integrity dye. Cells are immunophenotyped by staining with a fluorescent antibody panel to separate CD3+ T cells, CD3- non-T cells, CD4+ T helper cells, CD8+ T cytotoxic cells. The panel also includes 5 different T cell surface markers for T naive/memory/effector cell phenotyping: CD45RA, CD45RO, CD27 (long term survival), CD62L, and CD95. Optimized workflow enables the measurement of effector cytokines secreted by activated T cells, including pro-inflammatory cytokine IFNγ and anti-inflammatory cytokine IL-10 in the same assay well.