Human L507 Array, Glass Slide by RayBiotech Inc.

Manufacturer RayBiotech Inc.  |   Model: AAH-BLG-1
RayBio® L-Series Human Antibody Array 507 Glass Slide Kit. Detects 507 Human Proteins. Suitable for serum, plasma and cell culture supernatants.


Human L507 Array, Glass Slide by RayBiotech Inc. product image
Human L507 Array, Glass Slide

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Product Feature

  • High density arrays
  • 507 Human proteins
  • Direct biotin labeling of proteins
  • High detection sensitivity
  • Accurate and reproducible
  • Affordable, quick and simple to use

Target Species

Human

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing
Kit Components
  • RayBio® L-Series Human 507 Antibody Array Glass Slide(s)
  • Dialysis Tubes
  • Labeling Reagent
  • Stop Solution
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Streptavidin-Conjugated Fluor
  • Adhesive Plastic Strips
  • Serum Buffer
  • Floating Dialysis Rack
  • 30 ml Centrifuge Tube
Other Materials Required
  • Distilled or de-ionized water
  • KCl, NaCl, KH2PO4 and Na2HPO4
  • Small plastic or glass containers
  • Orbital shaker or oscillating rocker
  • Beaker, stir plate and stir bar
  • 1 ml tube
  • Pipettors, pipette tips and other common lab consumables
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner
Protocol Outline
  1. Dialyze samples in PBS
  2. Biotinylate Samples
  3. Dialyze biotinylated samples using spin column
  4. Dry the glass slide
  5. Block array surface
  6. Incubate with Samples
  7. Incubate with Streptavidin-Conjugated Fluor
  8. Disassemble the glass slide
  9. Scan with a gene microarray laser scanner
  10. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Number of Targets Detected

507

Product Overview

Human L507 Array, Glass Slide by RayBiotech Inc. product image

Human L507 Array, Glass Slide

Manufacturer RayBiotech Inc.

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