Human Insulin ELISA by RayBiotech Inc.

Manufacturer RayBiotech Inc.  |   Model: ELH-Insulin
4.5
/
5.0
  |  2 reviews
RayBio® Human Insulin ELISA Kit for cell culture supernatants, plasma, and serum samples.


Human Insulin ELISA by RayBiotech Inc. product image
Human Insulin ELISA



Average Rating: 4.5
2 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2021

  • Organization: Adamas University



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Great results, loved the product, good luck
Rating: 4.0

  • Application Area: To measure the blood levels of human insulin

"Human Insulin ELISA Kit has been used to determine human insulin and pro-insulin concentration in serum. The product is very effective and easy to use. And the results are also accurate. And can test any type of sample like serum, plasma, cell culture supernatants and urine. There I got pre coated 96 well plate of fine quality. We can do many types of elisa in it. I gave it 4 out 5 for easy to use as the product is very easy to use but if you are not experienced enough then you can mess up as you need to be careful while doing elisa. Also the product is price effective"

Review date: 13 Feb 2022 | Human Insulin ELISA
  • Status:

    Reviewer

  • Member since: 2017

  • Organization: Sao camilo university



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results
Rating: 5.0

  • Application Area:Diabetes model

"Excellent product, great results. Very easy to handle!"

Review date: 20 Jul 2017 | Human Insulin ELISA

Product Features

Sensitivity
4 µIU/ml

Looking for higher sensitivity? Check out our new Ultrasensitive Biomarker Testing Service powered by SIMOA™ technology.

Detection Range
4 µIU/ml - 300 µIU/ml
Recommended Dilution (serum & plasma)
2 - 10 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

This ELISA kit shows no cross-reactivity with the following cytokines tested: human BDNF, BLC, ENA-78, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TNF-alpha, TNF-beta, TPO, VEGF.
Species Detected
Human
Protein Name & Synonyms
Insulin
Accession Number
P01308
Gene Names
INS
Gene ID
3630

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.