Human/Mouse/Rat Phospho-JNK (T183/Y185) and Total JNK ELISA by RayBiotech Inc.

Manufacturer RayBiotech Inc.  |   Model: PEL-JNK-T183-T  |  Available Worldwide
RayBio® Human/Mouse/Rat Phospho-JNK (T183/Y185) and Total JNK ELISA Kit. This assay semi-quantitatively measures phosphorylated JNK (Thr183/Tyr185) and Total JNK in lysate samples.


Human/Mouse/Rat Phospho-JNK (T183/Y185) and Total JNK ELISA by RayBiotech Inc. product image
Positive Control
Request Pricing

Receive your quote directly from the manufacturer.



0 Scientists have reviewed this product


Write the First Review

No Reviews

Introduction

RayBio® Phospho- JNK (T183/Y185) ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human, mouse and rat cell lysates. By determining phosphorylated JNK protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.

This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human, mouse and rat phospho-JNK. An anti-pan JNK antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and JNK present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-JNK (T183/Y185) antibody is used to detect phosphorylated JNK. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of JNK (T183/Y185) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Product Features

  • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
  • Screen numerous different cell lysates without performing a Western Blot analysis
  • Minimal hands-on time, convenient, and non-radioactive material

Specificity

The antibody pair provided in this kit recognizes human, mouse, or JNK phosphorylated at sites Threonine-183 and Tyrosine-185 and total (pan) JNK.

Target Protein Information

Synonyms
Mitogen-activated protein kinase 8 (MAP kinase 8) (MAPK 8) (EC 2.7.11.24) (JNK-46) (Stress-activated protein kinase 1c) (SAPK1c) (Stress-activated protein kinase JNK1) (c-Jun N-terminal kinase 1)
Accession Number
Gene Names
MAPK8 JNK1 PRKM8 SAPK1 SAPK1C
Gene ID
5599

Species Detected

Human, Mouse, or Rat

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • li id="">Anti-Phospho Antibody
  • Anti-Pan Antibody
  • HRP-Conjugated Secondary Antibody
  • Streptavidin-Conjugated HRP
  • Assay Diluent
  • TMB One-Step Substrate
  • Stop Solution
  • Lysis Buffer
  • Positive Control Sample
Other Materials Required
  • Distilled or deionized water
  • 100 ml and 1 liter graduated cylinders
  • Tubes to prepare sample dilutions
  • Protease and Phosphatase inhibitors
  • Precision pipettes to deliver 2 µl to 1 ml volumes
  • Adjustable 1-25 ml pipettes for reagent preparation
  • Benchtop rocker or shaker
  • Microplate reader capable of measuring absorbance at 450 nm
Protocol Outline
  1. li id="aeaoofnhgocdbnbeljkmbjdmhbcokfdb-mousedown">Prepare all reagents and samples as instructed in the manual.
  2. Add 100 µl of sample or positive control to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 µl of prepared primary antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared 1X HRP-Streptavidin to each well.
  7. Incubate 1 h at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

Upon receipt, the kit should be stored at –20°C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4°C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge, and store at –20°C. Item D, store at 2-8°C for up to one month (store at -20°C for up to 6 months, avoid repeated freeze-thaw cycles). Reconstituted Positive Control (Item K) should be stored at -70°C.

Product Overview

Human/Mouse/Rat Phospho-JNK (T183/Y185) and Total JNK ELISA by RayBiotech Inc. product image

Human/Mouse/Rat Phospho-JNK (T183/Y185) and Total JNK ELISA

Manufacturer RayBiotech Inc.  |  Available Worldwide

Be the first to review this product