High Resolution ChIP-seq Library Prep kit
Diagenode's High Resolution Library Preparation Kit significantly enhances the resolution of transcription factor binding sites to single nucleotide levels.Using an optimized, proprietary protocol using an exonuclease to digest protein-bound DNA from the 5' end to within a few nucleotides of the protein binding site, the precise location of transcription factor-bound DNA can be determined. The High Resolution Library Preparati…
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Diagenode's High Resolution Library Preparation Kit significantly enhances the resolution of transcription factor binding sites to single nucleotide levels.
Using an optimized, proprietary protocol using an exonuclease to digest protein-bound DNA from the 5' end to within a few nucleotides of the protein binding site, the precise location of transcription factor-bound DNA can be determined. The High Resolution Library Preparation Kit provides a much higher resolution than in ChIP-seq and thus more accurately determines binding sites.
Features:
- Ultra-precise: Provides exact binding sites for transcription factors
- Higher resolution and improved signal-to-noise ratio
- Optimized for use with the iDeal ChIP-seq kit for Transcription Factors
- Compatible with Illumina sequencing
- Multiplex up to 8 samples
Bioinformatics Pipeline for ChIP-Seq Analyses
This poster presents a modular bioinformatics pipeline which was developed specifically for the analysis of ChIP-seq data, and can be easily modified and extended to accommodate other high-throughput sequencing and epigenetic applications, such as RNAseq or methylation analysis and other custom data processes.
