High Pure miRNA Isolation Kit by MilliporeSigma, a business of Merck KGaA Darmstadt Germany

Manufacturer MilliporeSigma, a business of Merck KGaA Darmstadt Germany  |   Model: 05080576001 ROCHE  |  Available Worldwide
The High Pure miRNA Isolation Kit purifies and enriches small RNAs, such as microRNA (miRNA) from animal cells and tissue samples (including formalin-fixed, paraffin-embedded sections) or plant material


High Pure miRNA Isolation Kit by MilliporeSigma, a business of Merck KGaA Darmstadt Germany product image
Kit for 50 isolations

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The High Pure miRNA Isolation kit rapidly purifies RNA that is suitable for direct use in many downstream applications:

  • Northern blotting
  • cDNA synthesis
  • Primer extension
  • miRNA array hybridization
  • Relative quantification of miRNA with RT-PCR using, for example, the LightCycler® 480 System

 

Features and Benefits

  • Isolate DNA-free miRNA ideal for qualitative and quantitative reverse transcription; ideal for qualitative and quantitative reverse transcription.
  • Obtain excellent performance and linearity in RT-PCR.
  • Generate stable, highly pure miRNAs using a simple, efficient protocol.
  • Purify miRNA without using hazardous organic solvents. Avoid phenol/chloroform steps required by other suppliers′ miRNA purification kits.
  • Choose one flexible kit for all your miRNA purifications. Use the same kit to purify small RNAs from a variety of sample types.

Quality

More than 60% recovery is obtained when 1 μg miRNA is added to106 K-562 cells and isolated with the one-column method, and more than 50% is recovered with the two-column method.

 

Product Overview

High Pure miRNA Isolation Kit by MilliporeSigma, a business of Merck KGaA Darmstadt Germany product image

High Pure miRNA Isolation Kit

Manufacturer MilliporeSigma, a business of Merck KGaA Darmstadt Germany  |  Available Worldwide

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