The ViaCount Assay provides rapid and reliable determinations of viability and total cell count on all Guava® Systems. Precise, accurate assessments can be made with a wide variety of cell lines, even those with unusual culture conditions or a tendency to aggregate.
A simple no-wash, mix-and-read procedure, the ViaCount Assay accurately determines absolute total cell counts, viability assessments, and apoptotic percentages with as little as 20 μL of sample.
The assay has proven to be more accurate than hemocytometer counting or impedence-based counting over a wide range of cell concentrations without the use of expensive reference beads. And, by providing data on all three types of cell populations in a single measurement, the Guava ViaCount Assay enables a more precise evaluation of culture conditions and viable cell populations so you can best optimize culture conditions. Precise, accurate cell counts provide more consistency for experiments that depend upon accurate determination of viable cells for success, such as ELISpot, cell-based fluorescence assays, or cytokine production assays.
Distinguishes viable, dead, and apoptotic cells
The ViaCount Assay distinguishes viable and non-viable cells based on differential permeabilities of two DNA-binding dyes in the Guava ViaCount® Reagent. The nuclear dye stains only nucleated cells, while the viability dye brightly stains dying cells. This proprietary combination of dyes enables the Guava ViaCount Assay to distinguish viable, apoptotic, and dead cells. Debris is excluded from results based on negative staining with the nuclear dye.