Member since: 2016
Organization: Beckman Coulter Inc.
Easy to use, compatible with human stem cell-based organoid culture.
Application Area: Embedding organoids for shipping
"GrowDex T is a transparent hydrogel that is ready-to-use after mixing with a cell culture medium of choice. We embedded organoids for mechanical protection during transport and found that GrowDex boosts cell viability under these circumstances without altering morphology or apparent cell differentiation. In addition, it was easily removed via cellulase enzyme, which digests growdex-cellulose, but leaves human tissues and cell-cell connections intact."
Member since: 2020
Organization: Medical University of Innsbruck
Very positive experiences and additionally optimization of our culturing processes!
Application Area:Examine viral interactions in a three dimensional human derived epithelial lung model
"As our working group has already shown before, the GrowDex products have a positive impact on the proliferation and differentiation of Normal Human Bronchial Epithelial cells (NHBE). We cultivate these epithelial cells and generate a three dimensional multi layered lung model under animal-free conditions. This model is a very good base to study host-pathogen interactions, especially in these days for studying the interaction of viral particles with human lung epithelium. At the beginning we used GrowDex to seed the cells in Transwells and so replace rat tail collagen. The GrowDex product is very easy in use and works excellently as extracellular matrix for the cells to grow in. Since this product showed positive results in proliferation and differentiation rates of NHBE cells we also decided to give the new GrowDex-T a try. Also this product shows the same advantages for our research and furthermore we could use it in a lower concentration, what makes it even cheaper. For now we grow the lung model for more than one and a half years in GrowDex products. This made it possible for us to save money, get rid of rat tail collagen as the last animal derived compound in our culturing process and even get better results concerning expansion and differentiation of cells. Not only that this product is very easy to mix and apply it also offers the big advantage that it can be digested with the GrowDase. This is another big advantage of GrowDex products, because this cellulose based hydrogel can be easily digested without harming the cells growing in it. So it is possible to resolve the cultivated cells out of the extracellular matrix and use them for further experiments. Besides all these positive impacts, the customer service from UPM Biochemicals is very friendly and helpful, making it even more joyful to order and work with their products!"
With GrowDex® hydrogels you can work through your entire HTS/HCS workflow form setting up your assays, screening your compounds, analysing your cells and moving to downstream processes, with high biological predictability and reproducibility, while saving time and costs. GrowDex®-T is a ready to use hydrogel that has been specifically developed to offer superior imaging properties, but without compromising on any of the advantages of our original hydrogel GrowDex.
Applications for GrowDex® Hydrogels: