EnGen sgRNA Synthesis Kit by New England Biolabs Inc.

Manufacturer New England Biolabs Inc.  |  Available Worldwide
4.3
/
5.0
  |  1 reviews
The EnGen sgRNA Synthesis Kit,  S. pyogenes  provides a simple and quick method for transcribing high yields of sgRNA in a single 30-minute reaction, using the supplied reagents and target-specific DNA oligos designed by the user.


EnGen sgRNA Synthesis Kit by New England Biolabs Inc. product image
EnGen sgRNA Synthesis Kit

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Average Rating: 4.3
1 Scientist has reviewed this product

5 out of 5
Ease of use
3 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2020

  • Organization: Robigus Genetics, LLC



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    5 out of 5
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sgRNA made easy and cheap by NEB.
Rating: 4.3

  • Application Area: sgRNA synthesis for CRISPR

"The NEB kit for making one's own sgRNA is easy to use. Makes 17ug and only takes 30 minutes. All you have to supply is a 50nt DNA guide sequence. For $250 and ten uses, this kit is very affordable compared to ordering synthetic RNA kits. This kit is another home run for NEB."

Review date: 23 Jul 2020 | EnGen sgRNA Synthesis Kit

The EnGen sgRNA Synthesis Kit, S. pyogenes provides a simple and quick method for transcribing high yields of sgRNA in a single 30-minute reaction, using the supplied reagents and target-specific DNA oligos designed by the user.

  • Rapid generation of microgram quantities of sgRNA
  • Complete workflow of less than one hour
  • Simple protocol requires only a user-supplied ~55 nucleotide ssDNA target-specific oligonucleotide
  • For help with oligo design, try EnGen sgRNA Template Oligo Designer - now included in the NEBioCalculator® Tool
  • Compatible with EnGen Spy Cas9 NLS, EnGen Spy Cas9 Nickase, EnGen Spy dCas9 (SNAP-tag®)