Member since: 2020
Organization: Robigus Genetics, LLC
sgRNA made easy and cheap by NEB.
Application Area: sgRNA synthesis for CRISPR
"The NEB kit for making one's own sgRNA is easy to use. Makes 17ug and only takes 30 minutes. All you have to supply is a 50nt DNA guide sequence. For $250 and ten uses, this kit is very affordable compared to ordering synthetic RNA kits. This kit is another home run for NEB."
The EnGen sgRNA Synthesis Kit, S. pyogenes provides a simple and quick method for transcribing high yields of sgRNA in a single 30-minute reaction, using the supplied reagents and target-specific DNA oligos designed by the user.