End-It™ DNA End-Repair Kit by Epicentre Technologies Corp.

5.0
/
5.0
  |  1 reviews
Repair sheared, nebulized, or restriction enzyme-digested genomic DNA for: preparation of templates for next-gen sequencing; or shotgun library preparation. The End-It™ DNA End-Repair Kit converts DNA containing damaged or incompatible 5´- and/or 3´-protruding ends to 5´-phosphorylated, blunt-ended DNA. The end-repaired DNA can be rapidly ligated into a cloning vector or to next-gen sequencing adaptors, using the Fast-Link™ DNA Ligation Kit. The End-It DNA End-Repair Kit contains reagents for 20... Read more


End-It™ DNA End-Repair Kit by Epicentre Technologies Corp. product image
End-It™ DNA End-Repair Kit

Epicentre Technologies Corp.

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Average Rating: 5.0
1 Scientist has reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2009

  • Organization: DANA-FARBER CANCER INSTITUTE



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Rating: 5.0

"A great and efficient reagent to clean up your CHIP'd products to prepare for next sequencing libraries."

Review date: 18 May 2009 | End-It™ DNA End-Repair Kit
Repair sheared, nebulized, or restriction enzyme-digested genomic DNA for: preparation of templates for next-gen sequencing; or shotgun library preparation.

The End-It™ DNA End-Repair Kit converts DNA containing damaged or incompatible 5´- and/or 3´-protruding ends to 5´-phosphorylated, blunt-ended DNA. The end-repaired DNA can be rapidly ligated into a cloning vector or to next-gen sequencing adaptors, using the Fast-Link™ DNA Ligation Kit.

The End-It DNA End-Repair Kit contains reagents for 20 or 50 end-repair reactions (repair of up to 100 µg or 250 µg of genomic DNA, respectively).

Benefits include:

• The repaired DNA is blunt-ended and 5´-phosphorylated for immediate blunt-end ligation. 
• The high specific activity of the End-Repair Enzyme Mix provides complete conversion of protruding ends to 5´-phosphorylated, blunt-ended DNA.