Repair sheared, nebulized, or restriction enzyme-digested genomic DNA for: preparation of templates for next-gen sequencing; or shotgun library preparation.
The End-It™ DNA End-Repair Kit converts DNA containing damaged or incompatible 5´- and/or 3´-protruding ends to 5´-phosphorylated, blunt-ended DNA. The end-repaired DNA can be rapidly ligated into a cloning vector or to next-gen sequencing adaptors, using the Fast-Link™ DNA Ligation Kit.
The End-It DNA End-Repair Kit contains reagents for 20 or 50 end-repair reactions (repair of up to 100 µg or 250 µg of genomic DNA, respectively).
• The repaired DNA is blunt-ended and 5´-phosphorylated for immediate blunt-end ligation.
• The high specific activity of the End-Repair Enzyme Mix provides complete conversion of protruding ends to 5´-phosphorylated, blunt-ended DNA.
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