Dynabeads™ MyOne™ Silane by Thermo Fisher Scientific
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Status:
Advanced Reviewer
Member since: 2018
Organization: Broad Institute
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Makes simultaneous RNA isolation easier and happier!
Application Area: RNA isolation from cells and tissues
"I usually use the reagent forRNA isolation by magnetic separation. With combination of 96 well magnet, you can do the procedure in parallel and you don't need any centrifugation. Just repeat magnet separation. You can also treat with DNase on the beads and remove the enzyme by simple magnet separation. Additionally, you can make the elution volume quite low (< 10 µL) so it is very suitable for small starting material. I cannot do RNA isolation without the reagent!"
Review date: 02 Oct 2019 | Dynabeads™ MyOne™ Silane
For highly predictable and consistent extraction and isolation of nucleic acids from biological samples, following a simple magnetic separation protocol. This is a stand-alone product containing only Dynabeads® in a storage buffer.
Magnetic particles from alternative suppliers often have a random size range distribution, surface area and binding capacity. This could compromise the reproducibility of your results. With Dynabeads® MyOne™ SILANE you have ensured higher sensitivity, capacity and performance.
The small 1 µm Dynabeads® MyOne™ SILANE have an optimized silica-like surface chemistry and a high specific surface area, providing efficient kinetics and a high sensitivity in nucleic acid capture. Their uniform size and surface area also ensure reproducible results. The product holds reputable Dynal® high standards with respect to within- and between-lot reproducibility and automation ability.
Advantages:
- Highly predictable binding per mg of beads
- High sensitivity, allowing for a low detection limit
- For isolation of viral DNA⁄RNA, total RNA, genomic DNA, etc.
- Isolates both DNA and RNA
- Quicker than spin columns
- More cost-effective, yet performs to the same level as spin columns
- Automation-friendly (slow sedimentation rate + high magnetic mobility)
- Adjusted to customer requirements on a custom OEM basis
Applications:
- Isolation of total nucleic acids (DNA⁄RNA)from typical biological samples (e.g. serum⁄plasma, whole blood, etc.)
- Well suited for automated assays.
- Dedicated kits have been developed for isolation of viral nucleic acids and genomic DNA (available separately).
For Research Use Only. Not for use in diagnostic procedures
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