ddSEQ™ Single-Cell Isolator by Bio-Rad

The ddSEQ Single-Cell Isolator is part of the Illumina^® Bio-Rad^® Single-Cell Sequencing Solution, a platform that brings Bio-Rad's Droplet Digital™ technology and Illumina's market-leading sequencing expertise to single-cell gene expression studies.

The ddSEQ Single-Cell Isolator works with the Illumina^® Bio-Rad^® SureCell™ WTA 3' Library Prep Kit for the ddSEQ™ System. This technology utilizes disposable microfluidic cartridges to coencapsulate single cells and barcodes into subnanoliter droplets, where cell lysis and barcoding occur. RNA-Seq libraries are subsequently prepared and sequenced. Analysis is conducted via BaseSpace.

Features and Benefits

• Process hundreds to tens of thousands of cells per day
• Sensitive and unbiased characterization of transcriptional signatures
• Scalable solution to accommodate a wide range of experimental designs
• Agnostic to mammalian cell size, enabling unbiased profiling of diverse cell populations
• End-to-end single-cell sequencing solution with simple, powerful data analysis

Applications and Uses

• Assess cell-to-cell heterogeneity and identify new sub-populations
• Map cell trajectories
• Dissect transcriptional mechanics
• Infer gene regulatory networks
• Neurology research — normal brain function, neurological disease and psychological disorders
• Immunology research — B Cell and T Cell subunit functional analyses
• Cancer research — studies of cellular expansion of clonal cells in tumors and mutations that lead to differences in gene expression and drug response
• Stem cell research — studies to better understand development and differentiation, including reverse engineering tissues

Included Components

• ddSEQ Test Cartridges
• ddSEQ Cartridge Holder
• ddSEQ Single-Cell Isolator
• Power supply and power cord
• User manual and product documentation