Luciferin is essential in performing your bioluminescent assay – and the quality of your research will depend on the quality of your luciferin. That’s why PerkinElmer, the world leader in the area of in vivo preclinical imaging, offers high quality Luciferin at an affordable price.
Luciferin is a chemical substance found in the cells of various bioluminescent organisms. When luciferin is oxidized under the catalytic effects of luciferase and ATP, a bluish-green light is produced. Because the reaction is dependent on ATP, it allows researchers to determine the presence of energy or life. Firefly luciferin is a particularly good reporter for in vivo biophotonic imaging due to properties of its emission spectra.
Luciferin can be used in a number of ways. It can be used in a variety of in vitro assays, where the production of light can be monitored with either a luminometer or a scintillation counter. It can also be used to monitor light production in vivo, and can be monitored with a PerkinElmer IVIS® Imaging System. Because luciferin can penetrate cell membranes, it allows transformed cells to be monitored for luciferase activity.
D-Luciferin bioluminescent substrate has been validated in a number of biophotonic imaging applications using PerkinElmer's IVIS® Imaging Systems.