Status:
Advanced ReviewerMember since: 2016
Organization: University of Buenos Aires
Great bands using these gels
Application Area: Western Blots
"Great product! Easy to use and the blots are just beautiful. I got the nicest bands with these gels."
Status:
ReviewerMember since: 2017
Organization: King's College London
Very reliable product and easy to use.
Application Area:Analyse proteins in bacterial lysates
"We have been ordering these precast gels for over 5 years in our lab as they save us a bit of time compared to making our own gels. Also, because the outer plates are disposable, we don't have to worry about risking breaking them, as with lab made gels which have fragile plates. There are cheaper precast gels out there but we have had the best results with these gels."
Status:
ReviewerMember since: 2014
Organization: UW-Madison
These gels are consistent, produce great bands and are easy to use.
Application Area:Protein changes
"I have used these gels for over 15 years & recommend for any western blot. High quality, reproducible results and easy to use."
Status:
ReviewerMember since: 2016
Organization: University of California, San Francisco
Can't live without it.
Application Area:Western blots of various cancer cell line lysates
"Really easy to use. These gels are nice because the lane for the ladder is small but the wells hold 45uL as opposed to 30uL so that if you need to load more lysate than normal or the lysate is dilute. We also use the Trans-Blot Turbo Transfer System. The transfer pads are a bit expensive but we are thinking about utilizing other transfer stacks to become more economical."
Status:
ReviewerMember since: 2010
Organization: Univ Pittsburgh
Awesome gels
Application Area:Western blots
"These gels allows high reproducibility of results and beautiful outcome of the WB"
Status:
ReviewerMember since: 2014
Organization: University of New Hampshire
I would not use any other manufacturer's gels
Application Area:Analysis of proteins separated on 1D and 2D PAGE
"Highest band resolution and reproducible results, particularly for 2D gels that must be reliable quantitative."
Status:
ReviewerMember since: 2011
Organization: The Ohio State University
Application Area:for western blotting
"Results quality is better. Separation is very good. Blots are super clean. Price are reasonable"
The Criterion™ TGX™ (Tris-Glycine eXtended) precast gels for PAGE are based on the long shelf life TGX formulation. The TGX gels retain Laemmli-like separation characteristics using the standard sample and Tris/Glycine running buffers. The proteins in the gel can be separated in as little as 20 min at 300 V while maintaining low temperatures.
Benefits Include:
TGX gels are manufactured without SDS. The gels can be used to run proteins under nondenaturing conditions for subsequent analysis of their native conformation and activity by omitting SDS from the running buffer. Native analysis does not provide accurate molecular weight information. SDS is added to the sample and running buffer to create standard SDS denaturing electrophoresis conditions.
Criterion gels are packaged and sold in individual units: 1–2 gels can be run in the Criterion cell and 1–12 gels can be run in the high-throughput Criterion™ Dodeca™ cell.