The Corning® Epoxide Slide Starter Kit offers 10 Epoxide Coated Slides, 0.8 mL of Pronto!™ Long-Oligo/cDNA Hybridization Solution, and 0.8 mL Pronto!™ Short Oligo Hybridization Solution. This kit is being offered as a way of providing a low cost trial of critical components for the optimization of oligo-array production and the subsequent hybridization to labeled cDNA.
The Corning® Epoxide Slides work best when oligos are dissolved in 150 mM sodium phosphate, ph 8.5 to 9.0. A small amount of detergent may be added to increase spot size, depending on printing conditions and the type of pins used.
When Corning® Epoxide Coated Slides are used in conjunction with the Pronto!™ Universal Hybridization Kit, the following features are evident:
• Sensitivity to 1 pg target RNA in a 4 µg total RNA direct labeling reaction
• Interslide CVs below 10%
• Detection of low expression targets above extremely low backgrounds