Member since: 2019
Organization: PhenoVista Biosciences
Brings expanded capabilities and innovation to the lab.
Application Area: Sort 3D structures (spheroids, organoids) for cell culture and subsequent handling
"We are a service company (CRO) that does a lot of 3D cell culture work, and this instrument has dramatically changed our ability to work with 3D structures, generate high-quality and reproducible assays, and consistently select and sort structures for subsequent cell culture and fluorescence imaging work. The instrument is intuitive to learn, but we've found it most efficient to have dedicated personnel that are familiar with its operation. The availability of both bright-field and fluorescence options allow sorting of both live-cell structures (e.g. we've used it to build bright-field algorithms to select for 'healthy' 3D structures in subsequent assays) and tagged/labelled/fixed structures (e.g., for transfected/transduced structures)."
The CELL HANDLER provides an automated cell isolation system consisted with image-based selection and subsequent picking and transfer of the selected cells individually to appropriate destination plates/tubes by pipetting-like manner. Either single cells or organoids/spheroids, not only cells grown in liquid medium but also those grown in semi-solid medium (e.g. Matrigel®), are applicable for this automated cell isolation system.
Briefly, morphological/phenotypic features of cells are obtained automatically by high throughput bright-field and fluorescent image analysis, so that users can easily select cells based on several cellular properties and histograms, namely “Criteria Selection”. Subsequently, the 8 individual tips are capable of picking and transferring the selected cells gently and rapidly, resulting in lowering cellular damage during plating/harvesting.
Various types of microtiter plates and microtubes can be chosen as destination depending on the following analysis including cell-based assays, omics studies and cell line development. In addition, custom plates such as Organ-on-a-Chip are also available.
*MatrigelⓇ is a registered trademark of Corning inc.
*For research use only. Not for use in diagnostic or therapeutic procedures.