The BDNF Emax® ImmunoAssay Systems provide optimized reagents and a protocol for the sensitive and specific detection of brain-derived neurotrophic factor (BDNF). After an overnight coating of a 96-well plate, the specific protein is detected using an antibody sandwich format. The systems use a horseradish peroxidase-conjugated secondary antibody and a single-component TMB substrate for the final chromogenic detection of bound neurotrophic factor. Using this system, BDNF in tissue culture supern... Read more
BDNF Emax ImmunoAssay Systems
Average Rating: 2.8
2 Scientists have reviewed this product
Status:
Reviewer
Member since: 2007
Organization: Sepracor
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- Ease of use
- After sales service
- Value for money
Rating: 3.3
"Best in class."
Review date: 07 Jan 2011 | BDNF Emax ImmunoAssay Systems
Status:
Reviewer Member since: 2006
Organization: McMaster University
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- Ease of use
- After sales service
- Value for money
Rating: 2.3
Review date: 14 Dec 2010 | BDNF Emax ImmunoAssay Systems
"doesn't recognise rbdnf."
Review date: 14 Dec 2010 | BDNF Emax ImmunoAssay Systems
The BDNF Emax® ImmunoAssay Systems provide optimized reagents and a protocol for the sensitive and specific detection of brain-derived neurotrophic factor (BDNF). After an overnight coating of a 96-well plate, the specific protein is detected using an antibody sandwich format. The systems use a horseradish peroxidase-conjugated secondary antibody and a single-component TMB substrate for the final chromogenic detection of bound neurotrophic factor. Using this system, BDNF in tissue culture supernatants, tissue homogenates, plasma and urine can be quantitated in the range of 7.85-500pg/ml. Binding and recovery from mouse brain homogenates has not been fully characterized.
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