Member since: 2019
Organization: Szent Istvan University, Hungary
Good, easy to use kits.
Application Area: Flow cytometric microbiome analysis
"As I have to focus on flow cytometric microbiomes assays nowadays, the cell counting and calibration bead kits from Invitrogen make the lab settings easier."
The Bacteria Counting Kit accurately enumerates bacteria by flow cytometry. The kit includes the SYTO™ BC dye, which is also available separately (Cat no. S34855), a high-affinity nucleic acid stain that easily penetrates both gram-negative and gram-positive bacteria, producing an exceptionally bright green-fluorescent signal. The kit also includes a calibrated suspension of polystyrene microspheres. Signals from both the stained bacteria and the beads are easily detected in the green fluorescence channel and can be distinguished on a plot of forward scatter versus fluorescence. The density of the bacteria in the sample can be determined from the ratio of bacterial signals to microsphere signals in the cytogram.