Member since: 2019
Organization: BIAL, Portela & Ca
ATP quantification with good repeatability and sensitivity.
Application Area: Quantify ATP content in cultured cells
"Have been using ATPlite for years to quantify ATP levels in cultured cells (viability assays). The kit is really simple to use and fast (only a few steps) and delivers good repeatability and sensitivity."
ATPLite is an Adenosine TriPhosphate (ATP) monitoring system based on firefly (Photinus pyralis) luciferase.
Revvity ATPLite is a luminescence assay is thealternative to colorimetric, fluorometric and radioisotopic assays for the quantitative evaluation of proliferation and cytotoxicity of cultured mammalian cells. ATP monitoring can be used to assess the cytocidal, cytostatic and proliferative effects of a wide range of drugs, biological response modifiers and biological compounds.
The major advantages of the ATPLite system are high sensitivity, excellent linearity, simplicity, fast results and the lack of cell harvesting or separation steps.
Furthermore, the Revvity ATPLite™ Constant Quanta™ assay system produces a long lived glow type signal with a half-life of greater than five hours, therefore a special luminometer with injectors is not required. The ATPLite kit is ideal for use with the Revvity TopCount® Microplate Scintillation and Luminescence Counter and the LumiCount® Microplate Reader in both 96- and 384-well microplates.