ASSIST PLUS

Cutting-edge research in the life sciences industries is increasingly complex, demanding ever-greater precision. Automated liquid handling is a key driver of increased precision and efficiency in many laboratory processes.

In this second edition SelectScience guide, we investigate several automated liquid handling solutions from INTEGRA.

Download this SelectScience guide to explore:

• Streamlining DNA purification
• Optimizing PCR and sequencing prep
• Cutting-edge genome sequencing automation
• Automation in routine assays
• Simplifying serial dilution
• Product features and benefits

Resource details:

• Document type: SelectScience guide
• Page count: 87
• Read time: 130 mins
• Edition: 2nd

Discover how the ASSIST PLUS pipetting robot can take the hassle out of 16S metagenomic library preparation. Using INTEGRA’s ready-to-run VIALAB scripts and MAG module, researchers can automate key PCR and clean-up steps for up to 24 samples at a time. The result is a faster, more reliable workflow that reduces hands-on time, improves consistency, and helps generate high-quality sequencing data with ease.

A serial dilution is the step-wise reduction of a concentration using a constant dilution factor and similar volumes. This highly standardized procedure has applications in drug discovery, microbiology, and molecular biology.

In this application note, INTEGRA discusses how to perform serial dilutions with optimized liquid handling parameters to prevent common issues leading to accumulated errors during inter-dependent steps.

Automated western blotting is a cutting-edge technology revolutionizing traditional protein detection and analysis. Employing robotic systems, microscale separation, and advanced imaging technologies streamlines the labor-intensive and time-consuming process of western blotting. Automating the multiple workflow steps increases efficiency, making it an invaluable tool in various fields of biological research.

In this application note, INTEGRA describes how the ASSIST PLUS pipetting robot and D-ONE single channel pipetting module, together with the Simple Western Jess, automate all the liquid handling steps required to analyze 24 samples, providing a complete walk-away solution for western blot protocol
automation.

DNA clean-up methods are used to purify samples of DNA by removing any unwanted components. This is crucial for the success of downstream processes in genomics, biotechnology, molecular biology, clinical research and other fields of biology.

In this application note, INTEGRA describes how Zymo Research’s DNA Clean & Concentrator™ (DCC) MagBead kit can be used with the VOYAGER adjustable tip spacing pipette on the ASSIST PLUS pipetting robot to automate DNA purification and concentration, enabling high throughput processing.

DNA size selection with magnetic beads plays a significant role in molecular biology, employing specific ratios to capture and separate fragments by size.

This application note from Integra Biosciences demonstrates the accurate handling of magnetic beads for reproducible PCR product purification and DNA size selection.

The precise handling of liquids is essential to almost all scientific experiments but, in this advanced era, manual pipetting alone is not enough to keep up with the demands of most modern laboratories. The growing demand for increased workflow efficiency has inspired the development of innovative pipetting solutions that significantly enhance throughput.

In this expert guide, discover a comprehensive collection of valuable resources covering the successful integration of automated and semi-automated liquid handling technologies into four commonly used laboratory techniques.

This guide will also explain:

• How to accelerate your tissue kit automation
• How to revolutionize your sample transfer
• How to simplify reagent and sample aliquoting

Automating qPCR master mix preparation is often considered tedious due to the need to constantly change program set-up based on varying master mix volumes and sample numbers. This application note demonstrates how the D-ONE single channel pipetting module for the ASSIST PLUS pipetting robot is designed to simplify the programming of automated qPCR master mix preparation protocols down to a single step. Here, the D-ONE and ASSIST PLUS is used to verify a SYBR® Green qPCR protocol prepared directly in a divided reservoir.

Overcoming high dead volume to save precious and expensive reagents is an important goal for laboratories. In this application note, INTEGRA Biosciences demonstrates the cost- and time-saving benefits when preparing master mixes with expensive SYBR® Green dye, TaqMan® probes and DNA polymerase reagents for multiplex qPCR using a divided reservoir.

Precise handling of liquids is essential to almost all scientific experiments, but in this modern age, manual pipetting alone is not enough to keep up with the requirements of most laboratories. Scientists now demand greater efficiency from their workflows – a trend that has been further intensified by the COVID-19 pandemic – inspiring the development of innovative pipetting solutions to dramatically improve throughput.

Download this application compendium to discover a range of topical resources that detail the effective incorporation of both automated and semi-automated liquid handling technologies across four essential lab techniques: cell culture, cell screening, sample extraction and purification, and PCR.

The eBook explores these core techniques using worked examples spanning a variety of pressing topics, including:

• Automated 3D cell-based assay solutions
• Precise and automated cell seeding
• Genotyping with the ASSIST PLUS pipetting robot
• Transfection-grade plasmid DNA purification
• Rapid purification of DNA from cell and tissue samples
• Automation of RNA extraction and purification
• Genotyping assay and sample screening
• Semi-automated rapid IVD amplification test
• Fully automated PCR assay set-up
• Rapid detection of HPV high-risk strains

Optimal pipette settings, including tip immersion depth, pipetting speeds and angles, maximize the consistency and reproducibility of the ELISA assay. Full automation capability, unmatched pipetting ergonomics, repeat dispense and multi-aspirate steps can be used for fast dispense and removal of reagents to speed up the process. Automatic tip change ensures to avoid any assay contaminations.

INTEGRA developed an innovative reservoir solution that features a SureFlo Array and a unique hydrophilic surface treatment offering incredibly low dead volumes.

Winners of the Scientists’ Choice Awards were revealed at the 2019 Pittcon conference and expo, held in Philadelphia, and saw scientists travel from all over the globe to be at the event and collect their well-deserved prizes. Among the winners were Thermo Fisher Scientific, for Best New Separations Product of 2018, WITec GmbH, for Best New Spectroscopy Product of 2018, and INTEGRA Biosciences, for Best New General Lab Product of 2018. In this video, SelectScience Assistant Editor Emily Adam reports on the highlights from the awards ceremony.

Discover how the ASSIST PLUS by Integra can open up automated pipetting for every lab, and effectively streamline routine pipetting tasks like serial dilutions and reagent additions.

Tahoe Therapeutics aims to use the speed and accuracy of these automated systems to generate a dataset fifty times larger than its already record-breaking Tahoe 100 million single-cell atlas

Genknowme uses the VOYAGER adjustable tip spacing pipettes to streamline epigenetics testing

Semi-automated cell seeding with ASSIST PLUS supports development of novel chemotherapies

INTEGRA’s range of ergonomic pipettes can help to maintain consistent pipetting angles

Streamlining workflows through automated pipetting solutions

Liquid handling solutions for flexible molecular diagnostics workflows

Recent survey provides insights into the new uses of pandemic pipetting platforms

We’ve highlighted reviews of laboratory automation products we think could help make your lab's journey to becoming more automated hassle-free

From tackling post-pandemic changes in the biopharmaceutical supply chain to addressing unmet therapeutic needs, simplified off-target effect analysis, and more, explore the latest advances in drug discovery with these free resources

Pipetting robot has helped an Arbovirus testing lab streamline its sample pooling and reduce the use of plastic labware