AssayMAP Bravo Protein Sample Prep Platform

In this application note, we demonstrate the potential of a state-of-the-art triple quadrupole LC/MS system for researchers to push the boundaries of protein biomarker discovery in this emerging frontier. A comprehensive targeted quantification assay for 1,000 proteins in human K562 cell lysate was developed using an enhanced nanoflow LC/TQ platform. The system integrates the Evosep One LC, Newomics UniESI ion source, and Agilent 6495D triple quadrupole mass spectrometer. The results show excellent quantification performance, enabling reliable protein quantification at an extremely low amount, approximating single-cell level detection

In this application note, Agilent Technologies describes a method for automated MHC-I associated peptide enrichment using the Agilent AssayMAP Bravo platform with large-capacity (25 µL) cartridges for immunopeptidomics analysis.

Therapeutic monoclonal antibodies (mAbs) represent one of the fastest growing classes of protein-based drugs in the biopharmaceutical industry. Here, Agilent Technologies presents an integrated workflow for peptide mapping of a mAb, from automated sample preparation to data analysis, featuring an Agilent AssayMAP Bravo liquid-handling robot, the Agilent 1290 Infinity II LC system, the Agilent 6545XT AdvanceBio Q-TOF, and the Agilent MassHunter BioConfirm B.09 software.

In this brochure, Agilent Technologies introduces the Agilent and ProZyme sample preparation workflows that are designed to help push your glycoprotein analysis to new levels of performance.

Phosphopeptide enrichment is one of the most challenging sample preparation steps for LC/MS analysis due to the variation in reproducibility and selectivity caused by manual sample preparation. In this application note, Agilent Technologies performs an automated phosphopeptide enrichment with both qualitative and quantitative analysis using the Agilent AssayMAP Bravo platform and an LC/Q‑TOF system was implemented for the HUPO Phosphopeptide Challenge.

In this application note, Agilent Technologies present a study to demonstrate an HCP analysis workflow that includes the AssayMAP Bravo platform for automated sample preparation, the 6545XT AdvanceBio LC/Q TOF for LC-MS/MS analysis, and the vendor-neutral software from proteinmetrics for data analysis.

In this application note, Agilent Technologies describe a method for the quantitative analysis of intact monoclonal antibodies (mAbs) from a biological matrix. The workflow is comprised of an Agilent AssayMAP Bravo platform coupled with an Agilent 6545XT AdvanceBio LC/Q-TOF.

Antibody-drug conjugates (ADCs) are a new class of biotherapeutics that represent a rapidly growing portion of the drug discovery pipeline in pharmaceutical companies. ADC sample preparation prior to analysis is typically done manually which can introduce variability and limit throughput. The Agilent AssayMAP Bravo is an automated sample preparation system that gives reproducibility, reduced hands-on time, scalability, simple method transfer, and it minimizes the possibility of human error.

Monoclonal antibody (mAb) based entities represent a rapidly growing class of biologics that require extensive characterization to obtain approval for clinical trials and subsequent market release. Accurate mass measurement is a challenging step in the analytical characterization of antibodies because of their large size and the presence of post-translational modifications such as glycosylation. These characteristics also make determining the location of modifications more complex.

Lab productivity is facing significant challenges, including rising demand, staffing shortages, and the need to maintain data quality while achieving high throughput. Discover how Agilent Technologies is transforming lab productivity with advanced automation solutions. In this video, Agilent Technologies’ Senior Product Managers Thomas Wu and Kevin McCann introduce the Bravo liquid handling platform and high-throughput mass spectrometry solutions such as the StreamSelect LC/MS System and RapidFire High Throughput MS. Learn how Agilent’s automation technologies help scientists enhance efficiency, reliability, and scalability in life sciences and genomic applications.

This video was filmed at SLAS2025.

This video features the Agilent Bravo Liquid Handling Platform combined with AssayMAP technology for an automated high-throughput solution. The AssayMAP cartridge allows for direct liquid transfer allowing you to accelerate biologics discovery and development. Auto equilibration and washing preserve assay integrity and ensure your data is accurate and reproducible. The Bravo Platform is controlled by the intuitive VWorks interface, allowing for simple and flexible method creation.

Watch this video to discover the Agilent Bravo Automated Liquid Handling Platform. This open automated platform leverages the high-throughput best-in-class liquid handling of the Bravo 96 AM with AssayMAP microchromatography technology to allow true chromatographic separation with precision flow control. Learn how the system can be configured for a monoclonal antibody purification and titre assay using a protein-A resin.

Watch this video to find out about two new products launched by Agilent Technologies. The BenchBot Robot is a compact automated solution that can be easily integrated with a range of devices, allowing end users to create flexible workstations. The new AssayMAP Bravo Platform is a fully automated solution for high-throughput protein sample preparation and purification. SelectScience.tv interview at LabAutomation 2011.

The automation platforms from Agilent Technologies deliver powerful solutions to meet customers needs

Watch this on-demand webinar to explore a complete workflow for the development of targeted biomarker measurements for precision medicine