Large-panel targeted proteomics with single-cell-equivalent sensitivity using nanoflow 6495D LC/TQ

In this application note, we demonstrate the potential of a state-of-the-art triple quadrupole LC/MS system for researchers to push the boundaries of protein biomarker discovery in this emerging frontier. A comprehensive targeted quantification assay for 1,000 proteins in human K562 cell lysate was developed using an enhanced nanoflow LC/TQ platform. The system integrates the Evosep One LC, Newomics UniESI ion source, and Agilent 6495D triple quadrupole mass spectrometer. The results show excellent quantification performance, enabling reliable protein quantification at an extremely low amount, approximating single-cell level detection