Direct Detection of SAH with a TR-FRET Readou The AptaFluor™ SAH Methyltransferase Assay uses a natural occurring aptamer, or riboswitch, that selectively binds with SAH, the invariant product of methyltransferase reactions. The exquisite affinity and selectivity of the riboswitch combined with a positive TR-FRET signal enable screening and profiling of histone methyltransferases with unparalleled sensitivity.
The most sensitive HTS methyltransferase assay available. Reduces enzyme usage and allows the use of physiological SAM concentrations as low as 100nM.
Outstanding reagent and signal stability reduces assay variability and allows for automated workflows.
Simple & HTS-Ready
The AptaFluor™ SAH Methyltransferase Assay is in a simple, HTS-ready mix & read format. Run your enzyme reaction, add quench reagent, add AptaFluor™ reagents, and read your plates.