AMPure XP - PCR Purification by Beckman Coulter Life Sciences
Status:
Advanced Reviewer
Member since: 2021
Organization: Strand
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- Ease of use
- After sales service
- Value for money
Excellent product for purifying your PCR products or NGS libraries
Application Area: Molecular Biology - Magnetic bead based purification of PCR products
"AMPure XP purification system helps us to obtain a purified DNA for NGS sequencing. It gets rid of all of all the PCR reagents like Primers, dNTPs, enzymes, etc. It yields a good concentration after purification and provides quality results on Tape profile/gel electrophoresis. The results are great both quantity and quality wise. It has this magnetic bead based principle which is convenient for pipetting. The procedure is also very simple. You just need to add the beads into your PCR final product. Incubate at RT for 5 min, keep on magnetic rack, once the beads are all on the side wall of tube, discard the entire liquid. And finally elute the purified DNA in elution buffer/TE buffer."
Review date: 04 Mar 2023 | AMPure XP - PCR Purification
Status:
Advanced Reviewer Member since: 2015
Organization: IRB Barcelona
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- Ease of use
- After sales service
- Value for money
Simple, scalable, high yield PCR/DNA fragment purification.
Application Area:PCR/DNA cleanup
"I have been using this product now for more than 10 years mainly due to the simplicity of the protocol, the high recovery rates and the scalability. Unlike columns with a minimum elution volume you can elute in very small volumes and the process is also easily adaptable (with correct magnet types) to processing 96 or 384 samples at a time."
Review date: 09 Aug 2019 | AMPure XP - PCR Purification
Status:
Reviewer Member since: 2012
Organization: University of California
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- Ease of use
- After sales service
- Value for money
Application Area:DNA purification
"Quick, very easy to use. Excellent size cut-off. Very reliable- trusted with precious samples."
Review date: 29 Feb 2012 | AMPure XP - PCR Purification
Status:
Reviewer Member since: 2011
Organization: Terry Fox Laboratory/B C Cancer Agency
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- Ease of use
- After sales service
- Value for money
"a bit expensive, but no lot-to-lot variability. a dependable product."
Review date: 16 Mar 2011 | AMPure XP - PCR Purification
The Agencourt AMPure XP system is a highly efficient, easily automated PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration, Agencourt AMPure XP can be easily used in manual and automated 96- or 384-well formats. Agencourt AMPure XP purification system recovers amplicons greater than 100 bp with consistency and high reproducibility. The recovery efficiency of the Agencourt AMPure XP kit for small and large amplicon sizes is superior to the traditional filtration PCR cleanup methods.
Features:
- High recovery of amplicons, greater than 100 bp
- Efficient removal of unincorporated dNTPs, primers, primer dimers, salts and other contaminants
- Stable PCR products post-cleanup: No PCR degradation after storage at 4° C for seven days
- Efficient recovery of double stranded and single stranded DNA templates
- Consistent recovery throughout the kit’s 12 month shelf life
- Faster manual and automated processing as compared to traditional post-PCR cleanup methods
Product Overview
