Ambion® Cells-to-CT™ Kits by Thermo Fisher Scientific

Manufacturer Thermo Fisher Scientific  |  Available Worldwide
4.2
/
5.0
  |  2 reviews
Ambion® Cells-to-CT™ Kits allow you to measure relative gene expression by real-time RT-PCR without having to purify RNA prior to amplification. Cells-to-CT™ Kits are available for both TaqMan® and SYBR® Green detection. Cells-to-CT™ kits provide a complete workflow for real-time qRT-PCR analysis directly from cultured cells without RNA purification. Featuring a unique method for lysing cultured cells while removing genomic DNA (gDNA) and preserving RNA integrity... Read more


Ambion® Cells-to-CT™ Kits by Thermo Fisher Scientific product image
Ambion® Cells-to-CT™ Kits

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Average Rating: 4.2
2 Scientists have reviewed this product

5 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2015

  • Organization: UKl Freiburg Neurologie



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
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Use it if you need to run RT-PCR from hundreds of samples
Rating: 4.0

  • Application Area: kit to get cDNA from cultured cells for high throughput RT-PCR assays.

"The Ambion® Gene Expression Cells-to-CT™ Kit has performed consistently. The performance is increased as compared to RNA purification, RT steps, and then qRT-PCR. There is less handling, less chance at mistakes. Because of this time savings and increase in sensitivity"

Review date: 25 Jan 2016 | Ambion® Cells-to-CT™ Kits
  • Status:

    Advanced Reviewer

  • Member since: 2015

  • Organization: Neurogeneration



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Rating: 4.3

  • Application Area:Real Time PCR with no need for RNA purification

"Cells-to-CT Kits allow you to measure relative gene expression by real-time RT-PCR without having to purify RNA prior to amplification. It is particularly useful if you don't have much sample because it lets you use as few as 10 cells (they even have single cell kits). I'm not all the way convinced with the assay reproducibility (sometimes there is fluctuation in the results) but when sample quantity is a limitation, it is a good choice."

Review date: 23 Jul 2015 | Ambion® Cells-to-CT™ Kits

Ambion® Cells-to-CT™ Kits allow you to measure relative gene expression by real-time RT-PCR without having to purify RNA prior to amplification. Cells-to-CT™ Kits are available for both TaqMan® and SYBR® Green detection.

Cells-to-CT™ kits provide a complete workflow for real-time qRT-PCR analysis directly from cultured cells without RNA purification. Featuring a unique method for lysing cultured cells while removing genomic DNA (gDNA) and preserving RNA integrity, the kits contain reverse transcription (RT) reagents for cDNA synthesis and either probe-based (TaqMan® probes) or dye-based (SYBR® Green dye) master mixes for real-time PCR analysis.

The 1-step and 2-step kits differ in that the 1-step kits combine reverse transcription and real-time PCR into a single step, significantly reducing the number of pipetting steps and protocol time.

 

Features:

  • Extraordinary ease and speed—96 samples for qRT-PCR in typically <10 min
  • No tedious RNA purification—no columns, heating, or centrifugation
  • Superior performance—designed for consistent accuracy, reproducibility, and sensitivity with 10–100,000 cells
  • Validated kit workflows—complete sets of reagents preoptimized to work efficiently right out of the box

Available Kits:

  • Cells-to-CT™ 1-Step TaqMan® Kit
  • Cells-to-CT™ 1-Step Power SYBR® Green Kit
  • TaqMan® Gene Expression Cells-to-CT™ Kit
  • Power SYBR® Green Cells-to-CT™ Kit
  • Single Cell-to-CT™ qRT-PCR Kit

Product Overview

Ambion® Cells-to-CT™ Kits by Thermo Fisher Scientific product image

Ambion® Cells-to-CT™ Kits

Manufacturer Thermo Fisher Scientific  |  Available Worldwide

4.2 / 5.0 | 2 reviews