Member since: 2015
Organization: UKl Freiburg Neurologie
Use it if you need to run RT-PCR from hundreds of samples
Application Area: kit to get cDNA from cultured cells for high throughput RT-PCR assays.
"The Ambion® Gene Expression Cells-to-CT™ Kit has performed consistently. The performance is increased as compared to RNA purification, RT steps, and then qRT-PCR. There is less handling, less chance at mistakes. Because of this time savings and increase in sensitivity"
Member since: 2015
Application Area:Real Time PCR with no need for RNA purification
"Cells-to-CT Kits allow you to measure relative gene expression by real-time RT-PCR without having to purify RNA prior to amplification. It is particularly useful if you don't have much sample because it lets you use as few as 10 cells (they even have single cell kits). I'm not all the way convinced with the assay reproducibility (sometimes there is fluctuation in the results) but when sample quantity is a limitation, it is a good choice."
Ambion® Cells-to-CT™ Kits allow you to measure relative gene expression by real-time RT-PCR without having to purify RNA prior to amplification. Cells-to-CT™ Kits are available for both TaqMan® and SYBR® Green detection.
Cells-to-CT™ kits provide a complete workflow for real-time qRT-PCR analysis directly from cultured cells without RNA purification. Featuring a unique method for lysing cultured cells while removing genomic DNA (gDNA) and preserving RNA integrity, the kits contain reverse transcription (RT) reagents for cDNA synthesis and either probe-based (TaqMan® probes) or dye-based (SYBR® Green dye) master mixes for real-time PCR analysis.
The 1-step and 2-step kits differ in that the 1-step kits combine reverse transcription and real-time PCR into a single step, significantly reducing the number of pipetting steps and protocol time.