ACQUITY UPC² System

Waters Acquity UPLC-MS/MS and Xevo TQ-S micro systems are designed to improve pesticide detection for ProVerde laboratories, helping scientists ensure customer safety for medical marijuana products.

This application note details the benefits of analytical quality-by-design (AQbD) and method lifecycle management (MLCM), being offered by Chromicen. The methods were used alongside a toolbox of Waters LC systems, chemistries, and software to solve customers' challenges with method development and validation. AQbD takes a holistic approach in order to allow an overall understanding of method performance. These are based on numerous multidimensional combinations and interactions of the factors involved in a product's lifecycle – from the idea, development transfer, marketing, and sale, to its discontinuation. The adoption of Waters ACQUITY UPLC and ACQUITY QDa technology has provided Chromicen with faster information, earlier in the method development process, contributing to their business success.

The Method Lifecycle Management (MLCM) approach is a holistic approach encompassing all activities, from method development to validation, routine use, change control and retirement of the method. This enhanced approach improves the method understanding and performance, leads to fewer out-of-specifications results (OOS), facilitates method transfer and has the potential to lessen the regulatory burden.

These slides showcase the higlights of a webinar presented by Dr. Horacio Pappa, Director of General Chapters, United States Pharmacopeia, and Dr. Alexander Schmidt, Founder and CEO, and Mijo Stanic, Cofounder, General Manager and Technical Director, Chromicent Pharmaceutical Services, that contains the method lifecycle management key concepts, its benefits to drug manufacturers and patients, and the current thinking of the United States Pharmacopeia (USP), U.S. Food and Drug Administration (FDA) and ICH.

Watch the full webinar on demand here>>

Since its introduction in 2004, each addition to the Acquity family has continuously pushed forward to enable you to find faster, simpler and better ways to support the work that you do.

The safe use of crop protection products is of paramount importance to the agricultural chemicals manufacturing industry. In this application note, ACQUITY UPC2 level enantioanalysis of five triazole fungicides and tandem quadrupole mass spectrometry were used for the trace in wheat grain and/or wheat straw. UltraPerformance Convergence ChromatographyTM (UPC2) applies the performance advantages of UPLC® to SFC, combining the use of supercritical CO2 with sub-2-μm particle columns.

UltraPerformance Convergence Chromatography™ (UPC2®) is a novel technology that applies the performance advantages of UPLC to supercritical fluid chromatography (SFC). Combining the use of supercritical CO2 with sub-2-μm particle columns, UPC2 represents an analysis technique that is orthogonal to reversed-phase LC and can be used to solve many troublesome separations that challenge conventional LC or GC analyses. It also generates less solvent waste as compared to liquid chromatography. This application note presents studies using UPC2 coupled with ultraviolet (UV) detection, mass spectrometry (MS), and evaporative light scattering (ELS) detection for the separation of triacylglycerols in tobacco, corn, sesame, and soybean seed oils.

In this application note, fast separations of three common carotenoids by UPC2 in less than 2 minutes are describes. A quantitative analysis of β-carotene dietary supplement capsules is also demonstrated. Carotenoids are natural pigments synthesized by plants and some microorganisms. For animals and humans, carotenoids play an important role in vision. Carotenoids also act as important antioxidants with a preventative effect for various diseases. Since carotenoids cannot be synthesized de novo in the human body, humans need to acquire them through diet and supplements.

Fatty acids, both free and as part of complex lipids, play a number of key roles in metabolism – as major metabolic fuel (storage and transport of energy), as essential components of all membranes, and as gene regulators. In LC/MS methods, although no sample derivatization is required, the runs typically involve labor-intensive and time-consuming sample preparation, and utilize toxic organic solvents, which are expensive to purchase and dispose. As described in this application note, the analysis of fatty acids in the free form instead of FAME derivatives results in easier and faster sample preparation. The organic phase extract containing all the FFA can be injected directly into the system, which results in significant savings in sample preparation and analysis time, solvent costs, and solvent waste disposal.

This application note demonstrates a fast separation of vitamin K1 trans and cis isomers and menatetrenone (MK-4), a common form of vitamin K2, by UPC2 in less than three minutes on an ACQUITY UPC2 HSS C18 SB Column. Vitamin K1 (phylloquinone) is an essential human nutrient produced in plants, especially green leafy vegetables. The vitamin K1 in natural products exists mainly as the trans form, while the vitamin K1 used in food supplementation is often synthetic K1, which may contain appreciable amounts of the cis form. The trans-vitamin K1 is bioactive, while the cis-K1 is not. It is highly desirable to separate the trans- and the cis-vitamin K1 isomers to truly evaluate the nutritional value of the supplement ingredient.

Berries of schisandra (Shisandra chinensis) have been widely used for medicinal purposes in traditional Chinese medicines for centuries. The berries contain a wide variety of organic compounds with dibenzo[a,c]cyclooctadiene lignans being of particular interest. This application note demonstrates the feasibility of using the Prep100q SFC System as a tool for isolating schisandrin A from dry schisandra berries extract using supercritical fluid extraction (SFE).

Watch this video to learn about research utilizing supercritical fluid chromatography (SFC) to develop application methods for cosmetic, pharmaceutical and therapeutic applications. Work being conducted at the Université d'Orléans, France, is focused on bioactive molecules, which can be applied to a range of applications so the versatility of SFC allows the team to easily research a wide range of analytes.

Discover how SYNAPT and UPC2 separation technology from Waters is used to study the efficient use of petroleum and environmental effects of oil spills at the Kyungpook National University, South Korea. Find out how this lab analyzes the chemical structure and reactions of petroleum in the environment to help manage oil spills.

Chris Howson, Open Access Lab Manager, BioCity Laboratory Services, describes the analytical technologies available to scientists working in the pharmaceutical and life science industries at the BioHub, Alderley Park, UK, including Waters ACQUITY QDa® Mass Detector, ACQUITY UPLC® H-Class System and ACQUITY UPC2® System.

In this video, learn how ion mobility mass spectrometry can be used to investigate polyaromatic hydrocarbons, compounds found in crude oil, that can also be environmental contaminants. Discover how the resulting collision cross-section data is utilized to understand the structural chemistry of such compounds. ​

Discover how the Waters Centre of Innovation at Vanderbilt University is improving the drug discovery process by reducing the need for animal testing, using organs-on-chips. Find out how this innovative laboratory is using the Waters SYNAPT G2-Si Mass Spectrometer to collect crucial data quickly and precisely.

At ASMS 2015, Dr John Langley, Associate Professor in Mass Spectrometry at the University of Southampton, discussed how he keeps his lab at the cutting edge of separation science, with help from the ACQUITY UHPLC and UPC2 systems from Waters. In this video, he explains why he made the change from HPLC to UHPLC and how Open Access analysis has revolutionized his research.

Watch this video to hear Rohit Khanna, VP of Marketing at Waters Corporation, describe the demand for complete innovative solutions that maximize efficiency, technology and software capabilities. An example includes fast, easy analysis of N-Glycans without compromising on sensitivity using the ACQUITY QDa system. Such mass spectrometry innovations have now become available to new market areas such as food, environmental and clinical. Interview filmed by SelectScience at Pittcon 2015.

Watch this video to learn about recent advances in supercritical fluid chromatography instrumentation for the development of highly efficient analytical methods. To find out more, click on the website link below.

Watch this video to lean about a comprehensive investigation of the utilization of SFC/ESI positive mode MS for chiral and achiral bioanalytical studies. To find out more, click on the website link below.

Dr. John Langley, Head of Characterisation and Analytics in the Department of Chemistry at the University of Southampton, explains how the institute’s state-of-the-art mass spectrometry facilities are the first to provide Waters UPC² technology in an open access environment. Hear how UPC² super critical fluid chromatography is being used by synthetic chemists for fast analysis of new materials, and how Dr. Langley predicts a predominant role for the technology in the future, across a range of research applications including clinical lipidomics.

We look back at a selection of this year’s best interviews with some of the world's leading experts

A mass spec scientist, from a world-renowned clinical pathology laboratory, shares the secrets to their success in this exclusive SelectScience interview

In this Q&A from our popular webinar, learn about an analytical procedure implemented by the pharmaceutical industry and how it can benefit your lab

Laboratory provides access to cutting-edge analytical technologies which strengthen modern life and health sciences research

Research center for traditional chinese medicine modernization recognized for advancing quality control authentication and profiling methods

Learn how innovations from Waters keep this lab at the cutting edge

CGU’s Healthy Aging Research Center focuses on degenerative diseases affecting elderly human health

How the Acquity UPLC system can save you time, money and solvent consumption