ZipChip™

This eBook explores the power of the ZipChip microchip capillary electrophoresis–mass spectrometry (CE-MS) platform in simplifying charge variant analysis and accelerating biologic drug characterization. Featuring high-throughput, plug-and-play capabilities, ZipChip offers faster data acquisition and enhanced separation for therapeutic development.

As biopharmaceuticals evolve, achieving precise control over monoclonal antibody (mAb) quality is critical for therapeutic efficacy and regulatory compliance. Explore how using ZipChip Capillary Electrophoresis-Mass Spectrometry (CE-MS) for direct spent media analysis enables the efficient monitoring of mAb quality attributes during bioprocessing. By comparing continuous and bolus glucose feeding strategies in CHO cell cultures, learn how ZipChip CE-MS provides insights into molecular weight, charge, and glycoform heterogeneity, with minimal sample preparation. Enhanced product quality, characterized by reduced glycation and improved glycoform profiles, underscores the potential of this method for advancing bioprocess control.

Oligonucleotides (oligos) represent a distinct class of therapeutics that include RNA, DNA, and their structural analogs, and are effective against a wide range of disease conditions. In this application note, 908 Devices presents a CE/ MS analysis of oligos by ZipChip coupled to a QExactive HF mass spectrometer. This positive ESI mode method utilizes an MS-friendly background electrolyte (BGE), that eliminates the use of ion-pairing agents such as hexafluoro isopropanol or alkyl amines.

Ying Ge, Professor of Cell and Regenerative Biology and Chemistry at the University of Wisconsin discusses how easy ZipChip is to use in proteomics. The 908 Devices ZipChip platform prepares and separates a wide range of biological samples, then electrosprays them into a mass spec for analysis. With the process taking as little as a few minutes and increased separation quality, the kit is a desirable option for a host of biotherapeutic, metabolomic, and proteomic applications.

In this video, Dr. Dan Fabris, Harold S. Schwenk Sr. Distinguished Chair in Chemistry at the University of Connecticut, discusses his work developing technologies for the detection and characterization of RNA. A particular focus of Fabris is investigating the role of RNA modifications in the relationship between drugs of abuse and HIV. Fabris also describes how he’s working with 908 Devices to push boundaries within the field with capillary electrophoresis-mass spectrometry (CE-MS). This technology holds the potential for powerful insights into the RNA world, to discover novel targets for therapeutics but also for use as therapeutic itself.

In this video, SelectScience speaks with Michael MacCoss, professor of genome sciences at the University of Washington, and Will Thompson, principal scientist at 908 Devices. MacCoss describes his work developing technologies to quantitatively measure proteins in complex matrices, and how his collaboration with 908 Devices is aiming to improve the speed and robustness of these measurements using capillary electrophoresis-mass spectrometry (CE-MS). Thompson describes how this technology is addressing key challenges in proteomics, and its potential in diagnostics, investigating bacterial proteomes, and metabolite analysis. Both scientists discuss the importance that collaborations have in accelerating their work.

Key oral presentation highlights the company’s prototype microfluidic chips for multi-omics workflows