Products & ReviewSeparations

CIMmultus OH

A tool for chromatographic purification of LNPs as an alternative to filtration methods.

Buy Now
BIA Separations

Place your order directly with the manufacturer.

Ease of Use
After Sales Service
Value for Money
Be the first to leave a review

CIMmultus® OH is a hydroxyl-functionalized monolithic chromatography column designed for hydrophobic interaction chroamtography (HIC). Its non-specific, hydrophilic surface enables the gentle removal of impurities such as unencapsulated nucleic acids, ethanol from encapsulation and formulation buffers, and other process-related contaminants—without affecting LNP structure or cargo integrity. In a high-recovery manner, it achieves the same goals as TFF and dialysis, by collecting hihgly purified and concentrated particles. The added benefits of removing impurities elevates this method above the existing methods.


The column leverages monolith technology for convective flow, allowing for high flow rates and low backpressure, even with viscous formulations. This makes CIMmultus® OH particularly suitable for scalable, high-throughput LNP purification, from early development to commercial production. Its broad binding profile and mild operating conditions support platform approaches and method development across a range of LNP modalities.


Key Features & Benefits

  • A purification of LNPs beyond filtration to enhance LNP formulations.
  • Removes unencapsulated cargo, ethanol and formulation buffers to achieve buffer exchange and concentration.
  • Operates under mild buffer conditions to preserve LNP structure and cargo.
  • Produces particles of high quality.


Applications

  • Downstream processing of LNP formulations post-encapsulation.
  • Purifiying complex LNP Biopharmaceuticals for in vivo CAR-T and CRISPR applications.
  • Purifiying targeted LNPs.
  • Removal of unencapsulated mRNA, siRNA, or small molecules
  • Downstream processing of mRNA vaccines and gene therapy products
  • Process development and platform method evaluation
  • Scalable purification for clinical and commercial manufacturing

Product Overview

Links